Oct-4 Antibody (YA4160)(PBS only)

Cat. No.: HY-P84463A: Data Sheet User Guide for Antibodies
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Oct-4 Antibody (YA4160) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Oct-4.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

Oct-4 Antibody (YA4160) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Oct-4.

Host

Mouse

Molecular Weight

Predicted band size: 39 kDa;

Observed band size: 45 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human

SwissProt ID

Q01860

Gene ID

5460 [NCBI]

Immunogen

Purified recombinant fragment of human (AA: 136-360) expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-1:1000
FC FC: Flow Cytometry	1:200-1:400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:10000

Purity	affinity purified.	Conjugation	Non-conjugated
Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, pH 7.4.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 or SOX15 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency

Subcellular Localization：Cytoplasm; Nucleus

Expression：
Tissue_specificity:It is expressed in the developing brain. The highest expression levels are found in specific cell layers of the cerebral cortex, olfactory bulb, hippocampus, and cerebellum. Expression levels are lower in adult tissues.

Induction:Transcriptional activity is positively regulated by PKM (PubMed:18191611) . Upon cell differentiation, expression is repressed by NR6A1 (PubMed:26769970)

Isoforms & Post-Translational Modification：Q01860 has 2 isomers: Q01860-1: 38571 Da (predicted); Q01860-2: 30085 Da (predicted).
Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression;Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation;ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription

Subunit：Interacts with PKM. Interacts with WWP2. Interacts with UBE2I and ZSCAN10 (By similarity). Interacts with PCGF1 (PubMed:26687479). Interacts with ESRRB; recruits ESRRB near the POU5F1-SOX2 element in the NANOG proximal promoter; the interaction is DNA independent (By similarity). Interacts with ZNF322 (By similarity). Interacts with MAPK8 and MAPK9; the interaction allows MAPK8 and MAPK9 to phosphorylate POU5F1 on Ser-355 (By similarity). Interacts (when phosphorylated on Ser-355) with FBXW8 (By similarity). Interacts with FBXW4 (By similarity). Interacts with SOX2 and SOX15; binds synergistically with either SOX2 or SOX15 to DNA (By similarity). Interacts with DDX56 (By similarity)

Synonyms

OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4

Documentation

User Guide for Antibodies (1077 KB)