RAGE Antibody(YA6617)

Cat. No.: HY-P86924: Data Sheet User Guide for Antibodies
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RAGE Antibody(YA6617) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAGE.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

RAGE Antibody(YA6617) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to RAGE.

Host

Rabbit

Clonality

Recombinant,Monoclonal

Molecular Weight

Predicted band size: 43 kDa

Species Reactivity

Mouse

SwissProt ID

Q15109

Gene ID

177 [NCBI]

Immunogen

Synthetic peptide within human RAGE aa 350-390.

Application &
Dilution Ratio

Application	Dilution Ratio
mIHC mIHC: Multiplex Immunohistochemical	1:100

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Cell surface pattern recognition receptor that senses endogenous stress signals with a broad ligand repertoire including advanced glycation end products, S100 proteins, high-mobility group box 1 protein/HMGB1, amyloid beta/APP oligomers, nucleic acids, histones, phospholipids and glycosaminoglycans (PubMed:27572515, PubMed:28515150, PubMed:34743181, PubMed:35974093, PubMed:24081950). Advanced glycosylation end products are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes (PubMed:21565706). These ligands accumulate at inflammatory sites during the pathogenesis of various diseases including diabetes, vascular complications, neurodegenerative disorders and cancers, and RAGE transduces their binding into pro-inflammatory responses. Upon ligand binding, uses TIRAP and MYD88 as adapters to transduce the signal ultimately leading to the induction of inflammatory cytokines IL6, IL8 and TNFalpha through activation of NF-kappa-B (PubMed:21829704, PubMed:33436632). Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key pro-inflammatory mediators (PubMed:19386136). Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons (PubMed:19906677). ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space. Participates in endothelial albumin transcytosis together with HMGB1 through the RAGE/SRC/Caveolin-1 pathway, leading to endothelial hyperpermeability (PubMed:27572515). Mediates the loading of HMGB1 in extracellular vesicles (EVs) that shuttle HMGB1 to hepatocytes by transferrin-mediated endocytosis and subsequently promote hepatocyte pyroptosis by activating the NLRP3 inflammasome (PubMed:34743181). Binds to DNA and promotes extracellular hypomethylated DNA (CpG DNA) uptake by cells via the endosomal route to activate inflammatory responses (PubMed:24081950, PubMed:28515150). Mediates phagocytosis by non-professional phagocytes (NPP) and this is enhanced by binding to ligands including RNA, DNA, HMGB1 and histones (PubMed:35974093). Promotes NPP-mediated phagocytosis of Saccharomyces cerevisiae spores by binding to RNA attached to the spore wall (PubMed:35974093). Also promotes NPP-mediated phagocytosis of apoptotic cells (PubMed:35974093). Following DNA damage, recruited to DNA double-strand break sites where it colocalizes with the MRN repair complex via interaction with double-strand break repair protein MRE11 (By similarity). Enhances the endonuclease activity of MRE11, promoting the end resection of damaged DNA (By similarity). Promotes DNA damage repair in trophoblasts which enhances trophoblast invasion and contributes to placental development and maintenance (PubMed:33918759). Protects cells from DNA replication stress by localizing to damaged replication forks where it stabilizes the MCM2-7 complex and promotes faithful progression of the replication fork (PubMed:36807739). Mediates the production of reactive oxygen species (ROS) in human endothelial cells (PubMed:25401185)

Subcellular Localization：Cell membrane,Cell projection, phagocytic cup,Early endosome,Nucleus,Cell membrane,Secreted,Cell membrane

Expression：
Tissue_Specificity: Endothelial cells. Increased expression in pre-term labor and preeclampsia placentas compared to controls (PubMed:33918759)
Induction: Induced in T cells by antigen stimulation and by the S100B ligand (PubMed:22509345). Induced in trophoblasts by DNA damage (PubMed:33918759)

Isoforms & Post-Translational Modification：Q15109 has 10 isomers: Q15109-1: 42803 Da (predicted); Q15109-2: 36193 Da (predicted); Q15109-3: 37050 Da (predicted); Q15109-4: 39020 Da (predicted); Q15109-5: 13192 Da (predicted); Q15109-6: 44773 Da (predicted); Q15109-7: 41234 Da (predicted); Q15109-8: 35322 Da (predicted); Q15109-9: 38379 Da (predicted); Q15109-10: 41098 Da (predicted).
Phosphorylated on its cytoplasmic domain by PKCzeta/PRKCZ upon ligand binding (PubMed:21829704)丨Targeted by the ubiquitin E3 ligase subunit FBXO10 to mediate its ubiquitination and degradation

Subunit：Constitutive homodimer; disulfide-linked (PubMed:24081950)

Synonyms

Advanced glycosylation end product-specific receptor antibody; Ager antibody; MGC2235 antibody; RAGE_HUMAN antibody; Receptor for advanced glycosylation end products antibody;

Documentation

User Guide for Antibodies (1077 KB)