TAB2 Antibody (YA5074)

Cat. No.: HY-P85382: Data Sheet User Guide for Antibodies
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TAB2 Antibody (YA5074) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TAB2.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation

Description

TAB2 Antibody (YA5074) is a Mouse-derived and non-conjugated monoclonal antibody, targeting to TAB2.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 76 kDa;

Species Reactivity

Human

SwissProt ID

Q9NYJ8

Gene ID

23118 [NCBI]

Immunogen

Purified recombinant fragment of human TAB2 expressed in E. Coli.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:200-1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:50-500
IF-Tissue IF-Tissue: Immunofluorescence-Tissue	1:50-500
IP IP: Immunoprecipitation	1-2μg per 100-500μg Total protein
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:50-3000

Purity	affinity purified.	Conjugation	Non-conjugated
Modification	Unmodified

Appearance

Liquid

Formulation

Supplied in PBS with < 0.1% sodium azide and 0.1% gelatin.

Storage & Stability

Stored at 2-8°C for 1 year, do not freeze.

Shipping

Shipping with blue ice.

Background

Function：Adapter required to activate the JNK and NF-kappa-B signaling pathways through the specific recognition of 'Lys-63'-linked polyubiquitin chains by its RanBP2-type zinc finger (NZF) (PubMed:10882101, PubMed:11460167, PubMed:15327770, PubMed:22158122, PubMed:27746020, PubMed:33184450, PubMed:36681779). Acts as an adapter linking MAP3K7/TAK1 and TRAF6 to 'Lys-63'-linked polyubiquitin chains (PubMed:10882101, PubMed:11460167, PubMed:15327770, PubMed:22158122, PubMed:27746020). The RanBP2-type zinc finger (NZF) specifically recognizes Lys-63'-linked polyubiquitin chains unanchored or anchored to the substrate proteins such as RIPK1/RIP1 and RIPK2: this acts as a scaffold to organize a large signaling complex to promote autophosphorylation of MAP3K7/TAK1, and subsequent activation of I-kappa-B-kinase (IKK) core complex by MAP3K7/TAK1 (PubMed:15327770, PubMed:18079694, PubMed:22158122). Also recognizes and binds Lys-63'-linked polyubiquitin chains of heterotypic 'Lys-63'-/'Lys-48'-linked branched ubiquitin chains (PubMed:27746020). Regulates the IL1-mediated translocation of NCOR1 out of the nucleus (By similarity). Involved in heart development (PubMed:20493459)

Subcellular Localization：Membrane; Peripheral membrane protein; Endosome membrane; Peripheral membrane protein; Lysosome membrane; Peripheral membrane protein; Cytoplasm, cytosol

Expression：
Tissue_specificity:Widely expressed. In the embryo, expressed in the ventricular trabeculae, endothelial cells of the conotruncal cushions of the outflow tract and in the endothelial cells lining the developing aortic valves

Isoforms & Post-Translational Modification：Q9NYJ8 has 2 isomers: Q9NYJ8-1: 76494 Da (predicted); Q9NYJ8-2: 58131 Da (predicted).
Degraded in a lysosome-dependent manner following interaction with TRIM38;SUMOylated by TRIM60; leading to inhibition of MAPK/NF-kappaB activation and the innate immune response;Ubiquitinated; following IL1 stimulation or TRAF6 overexpression. Ubiquitination involves RBCK1 leading to proteasomal degradation. Ubiquitinated at Lys-611 by TRIM45 leading to proteasomal degradation (PubMed:36681779);Phosphorylated;(Microbial infection) Methylated at Cys-673 by enteropathogenic E.coli protein NleE or S.flexneri protein OspZ: methylation disrupts zinc-binding and ability to bind 'Lys-63'-linked ubiquitin, leading to NF-kappa-B inactivation

Subunit：Interacts with MAP3K7 and TRAF6 (PubMed:10882101). Identified in the TRIKA2 complex composed of MAP3K7, TAB1 and TAB2 (PubMed:11460167). Binds 'Lys-63'-linked polyubiquitin chains (PubMed:19675569, PubMed:19935683). Interacts with NCOR1 and HDAC3 to form a ternary complex (By similarity). Interacts (via C-terminal) with NUMBL (via PTB domain) (PubMed:18299187). Interacts (via the C-terminus) with DYNC2I2 (via WD domains) (PubMed:19521662). Interacts with RBCK1 (PubMed:17449468). Interacts with TRIM5 (PubMed:21512573). Interacts with TRIM38 (via B30.2/SPRY domain), leading to its translocation to lysosomes and degradation (PubMed:24434549). Interacts with ASB1; this interaction promotes TAB2 stability (PubMed:33431678)

Synonyms

TAB2; KIAA0733; MAP3K7IP2; TGF-beta-activated kinase 1 and MAP3K7-binding protein 2; Mitogen-activated protein kinase kinase kinase 7-interacting protein 2; TAK1-binding protein 2; TAB-2; TGF-beta-activated kinase 1-binding protein 2

Documentation

User Guide for Antibodies (1077 KB)