Topoisomerase II alpha Antibody (YA5740)

Cat. No.: HY-P86048: Data Sheet User Guide for Antibodies
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Topoisomerase II alpha Antibody (YA5740) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Topoisomerase II alpha.

For research use only. We do not sell to patients.

Size		Stock
50 μL		Get quote
100 μL		Get quote

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Documentation
References

Description

Topoisomerase II alpha Antibody (YA5740) is a Mouse-derived and non-conjugated IgG1 monoclonal antibody, targeting to Topoisomerase II alpha.

Host

Mouse

Clonality

Monoclonal

Molecular Weight

Predicted band size: 174 kDa;

Observed band size: 174 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Human, Mouse, Rat

SwissProt ID

P11388

Gene ID

7153 [NCBI]

Immunogen

Synthesized peptide derived from human Topoisomerase IIα AA range: 1400-1531

Application &
Dilution Ratio

Application	Dilution Ratio
IHC-P IHC-P: Immunohistochemistry-Paraffin	1:200-400
ELISA ELISA: Enzyme Linked Immunosorbent Assay	1:500-5000

Purity	affinity chromatography.	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG1

Appearance

Liquid

Formulation

Supplied in PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Shipping

Shipping with blue ice.

Background

Function：Key decatenating enzyme that alters DNA topology by binding to two double-stranded DNA molecules, generating a double-stranded break in one of the strands, passing the intact strand through the broken strand, and religating the broken strand (PubMed:17567603, PubMed:18790802, PubMed:22013166, PubMed:22323612). May play a role in regulating the period length of BMAL1 transcriptional oscillation (By similarity)

Subcellular Localization：Cytoplasm; Nucleus, nucleoplasm; Nucleus; Nucleus, nucleolus

Expression：
Tissue_specificity:This gene is expressed in the tonsils, spleen, lymph nodes, thymus, skin, pancreas, testes, colon, kidneys, liver, brain, and lungs (PubMed:9155056) . It is also found in high-grade lymphoma, squamous cell carcinoma of the lung, and seminoma (PubMed:9155056) .

Isoforms & Post-Translational Modification：P11388 has 4 isomers: P11388-1: 174385 Da (predicted); P11388-2: 177501 Da (predicted); P11388-3: 178712 Da (predicted); P11388-4: 182681 Da (predicted).
Phosphorylation has no effect on catalytic activity. However, phosphorylation at Ser-1106 by CSNK1D/CK1 promotes DNA cleavable complex formation;(Microbial infection) Deubiquitinated by Epstein-Barr virus BPLF1; leading to stabilized SUMOylated TOP2A trapped in cleavage complexes, which halts the DNA damage response to TOP2A-induced double-strand DNA breaks;SUMOylated

Subunit：Homodimer. Interacts with COPS5. Interacts with RECQL5; this stimulates DNA decatenation. Interacts with SETMAR; stimulates the topoisomerase activity (PubMed:18790802, PubMed:20457750). Interacts with DHX9; this interaction occurs in a E2 enzyme UBE2I- and RNA-dependent manner, negatively regulates DHX9-mediated double-stranded DNA and RNA duplex helicase activity and stimulates TOP2A-mediated supercoiled DNA relaxation activity (PubMed:12711669). Interacts with HNRNPU (via C-terminus); this interaction protects the topoisomerase TOP2A from degradation and positively regulates the relaxation of supercoiled DNA in a RNA-dependent manner (By similarity). Interacts with MCM3AP isoform GANP (PubMed:23652018). Interacts with ERCC6 (PubMed:26030138). Interacts with PLSCR1 (PubMed:17567603). Interacts with GCNA; this interaction allows the resolution of topoisomerase II (TOP2A) DNA-protein cross-links (By similarity). Interacts with POL1RA/RPA1 (via dock II) and UBTF in the context of Pol I complex; may assist Pol I transcription initiation by releasing supercoils occurring during DNA unwinding. Interacts with TPRN; TPRN interacts with a number of DNA damage response proteins, is recruited to sites of DNA damage and may play a role in DNA damage repair (PubMed:23213405)

Synonyms

DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme;

Documentation

User Guide for Antibodies (1077 KB)

References

[1]. /biology-dictionary/u-251-cell.html [Content Brief]