Auramine O 

Auramine O is a yellow fluorescent dye. Auramine O is used to detect amyloid fibrils. Auramine O promotes lung malignancy. Auramine O is also used to determine algal cell viability and stain acid-fast bacteria^{[1][2][3][4][5][6]}.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Auramine O staining of Tuberculosis bacilli^[3]
1. Place the slide on a staining rack and stain with Auramine O solution for 20 min
2. Rinse the slide with water and soak it in 0.5% acid alcohol for 3 min
3. Rinse with water and counterstain with 1% KMnO₄ solution for 1 min
4. Check the staining effect with an LED microscope with at least 40 fields of view
Note: Tuberculosis bacilli appear as yellow luminescent organisms in a dark field of view

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

303.84

C₁₇H₂₂ClN₃

2465-27-2

Solid

Light yellow to yellow

N=C(C1=CC=C(N(C)C)C=C1)C2=CC=C(N(C)C)C=C2.[H]Cl

Room temperature in continental US; may vary elsewhere.

RT, sealed storage, away from moisture and light

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

• [1]. Kommareddi S, et al. Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis. Hum Pathol. 1984 Nov;15(11):1085-9.  [Content Brief]

  [2]. Hosseinpour S A, et al. Use of metal composite MOF‐5‐Ag2O‐NPs as an adsorbent for the removal of Auramine O dye under ultrasound energy conditions. Applied Organometallic Chemistry, 2018, 32(2): e4007.

  [3]. Gizaw N, et al. The yield of Auramine O staining using led microscopy with bleach treated sputum samples for detection of pulmonary tuberculosis at St. Peter tuberculosis specialized hospital, Addis Ababa, Ethiopia. J Clin Tuberc Other Mycobact Dis. 2019 Dec 12;18:100140.  [Content Brief]

  [4]. Amdursky N, et al. Auramine-O as a fluorescence marker for the detection of amyloid fibrils. J Phys Chem B. 2012 Nov 15;116(45):13389-95.  [Content Brief]

  [5]. Tung JC, et al. Auramine O, an incense smoke ingredient, promotes lung cancer malignancy. Environ Toxicol. 2017 Nov;32(11):2379-2391.  [Content Brief]

  [6]. Hawes I, et al. Use of the fluorochrome Auramine O for determination of cell viability in filamentous and thalloid algae. Phycologia, 1989, 28(4): 518-520.