BODIPY 505/515-8-C3-COOH 

BODIPY 505/515-8-C3-COOH is a green fluorescing derivative, as a fluorescent dye for imaging lipid droplets in nannochloropsis. BODIPY 505/515-8-C3-COOH can be used for the research of flow cytometric high-throughput screening and cell sorting^[1].

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Labeling of Cells
1. Dissolve BODIPY 505/515-8-C3-COOH in DMSO at a concentration of 4 mg/mL, then dilute to different working stock concentrations with DMSO.
2. Add an appropriate amount of BODIPY 505/515-8-C3-COOH working stock solution to the cell suspension to achieve a final DMSO volume fraction of 2-10% (v/v) and a final BODIPY 505/515-8-C3-COOH concentration of 0.8-4 μg/mL.
3. After mixing the cells and the dye thoroughly, incubate in the dark for 15 min, then detect using a flow cytometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

334.17

C₁₇H₂₁BF₂N₂O₂

878674-84-1

Solid

Orange to red

515

505

O=C(CCCC1=C2C(C)=CC(C)=[N]2[B+3]([F-])([N-]3C1=C(C)C=C3C)[F-])O

Room temperature in continental US; may vary elsewhere.

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

• [1]. ChristianSüdfeld, et al. Optimization of high-throughput lipid screening of the microalga Nannochloropsis oceanica using BODIPY 505/515. Algal Research,