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  3. Fluorescent Dye
  4. BODIPY-FL

BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm.

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BODIPY-FL

BODIPY-FL Chemische Struktur

CAS. Nr. : 165599-63-3

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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BODIPY-FL Related Antibodies

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Beschreibung

BODIPY-FL is a green fluorescent dye that can be used to label probes or primers. BODIPY-FL fluorescence can be quenched after interacting with uniquely positioned guanine, making it useful for quantifying specific DNA or RNA molecules. The maximum absorption wavelength of BODIPY-FL is 505 nm, and the maximum emission wavelength is 513 nm[1][2][3][4].

Cellular Effect
Cell Line Type Value Description References
A2780 EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A2780 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
A-375 EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human A375 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
FaDu EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human FADU cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
HT-29 EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human HT-29 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
MCF7 EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against human MCF7 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
NIH3T3 EC50
>30 μM
Compound: 7; BODIPY FL
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
Cytotoxicity against mouse NIH/3T3 cells assessed as reduction in cell viability incubated for 96 hrs by SRB assay
[PMID: 31718946]
In Vitro

BODIPY-FL-labeled sphingolipid analogs can be used to investigate sphingolipid internalization, transport, and endocytosis in mouse embryonic stem cells[3].
BODIPY-FL-labeled monoterpenes can quickly penetrate bacteria, mammalian, and fungal cells, allowing for the detection of characteristics of a broad spectrum of Gram-positive and Gram-negative bacteria, as well as pathogenic fungi[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

BODIPY-FL Related Antibodies
Molekulargewicht

292.09

Formel

C14H15BF2N2O2
CAS. Nr.
Appearance

Solid

Color

Brown to red

Emission (Em)

516
Excitation (Ex)

495
SMILES

O=C(CCC1=CC=C2C=C3C(C)=CC(C)=[N]3[B+3]([F-])([N-]21)[F-])O
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 100 mg/mL (342.36 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.4236 mL 17.1180 mL 34.2360 mL
5 mM 0.6847 mL 3.4236 mL 6.8472 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation

Purity: 99.15%

SDS (393 KB)

COA (254 KB) HNMR (263 KB) LCMS (84 KB)

Handling Instructions (2659 KB)
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.4236 mL 17.1180 mL 34.2360 mL 85.5901 mL
5 mM 0.6847 mL 3.4236 mL 6.8472 mL 17.1180 mL
10 mM 0.3424 mL 1.7118 mL 3.4236 mL 8.5590 mL
15 mM 0.2282 mL 1.1412 mL 2.2824 mL 5.7060 mL
20 mM 0.1712 mL 0.8559 mL 1.7118 mL 4.2795 mL
25 mM 0.1369 mL 0.6847 mL 1.3694 mL 3.4236 mL
30 mM 0.1141 mL 0.5706 mL 1.1412 mL 2.8530 mL
40 mM 0.0856 mL 0.4280 mL 0.8559 mL 2.1398 mL
50 mM 0.0685 mL 0.3424 mL 0.6847 mL 1.7118 mL
60 mM 0.0571 mL 0.2853 mL 0.5706 mL 1.4265 mL
80 mM 0.0428 mL 0.2140 mL 0.4280 mL 1.0699 mL
100 mM 0.0342 mL 0.1712 mL 0.3424 mL 0.8559 mL
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BODIPY-FL Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

BODIPY-FL165599-63-3Fluorescent Dyefluorescent dyeprobeprimermonoterpenoidbacteriapathogenic fungiInhibitorinhibitorinhibit

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