C2 Ceramide (Synonyms: Ceramide 2)

Name: C2 Ceramide
Brand: MedChemExpress
SKU: HY-101180
Rating: 5.0 (2 reviews)

Cat. No.: HY-101180 Purity: 99.85%: Data Sheet
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C2 Ceramide (Ceramide 2) is the main lipid of the stratum corneum and a protein phosphatase 1 (PP1) activator. C2 Ceramide activates PP2A and ceramide-activated protein phosphatase (CAPP). C2 Ceramide induces cells differentiation, autophagy and apoptosis, inhibits mitochondrial respiratory chain complex III. C2 Ceramide is also a skin conditioning agent that protects the epidermal barrier from water loss.

For research use only. We do not sell to patients.

CAS No. : 3102-57-6

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 2 publication(s) in Google Scholar

Other Forms of C2 Ceramide:

(E/Z)-C2 Ceramide Get quote

2 Publications Citing Use of MCE C2 Ceramide

RT-PCR

: C2 Ceramide purchased from MedChemExpress. Usage Cited in: Food Funct. 2025 Aug 11;16(16):6422-6433. [Abstract]; Western blot analysis for phosphorylation of proteins in NF-κB and MAPK signaling pathways in THP-1 cells treated with BCCY-1 in the presence and absence of C2 ceramide (20 μM, 1-3 h).

: C2 Ceramide purchased from MedChemExpress. Usage Cited in: Food Funct. 2025 Aug 11;16(16):6422-6433. [Abstract]; The mRNA expressions of MCP-1 in BCCY-1-treated THP-1 cells incubated with or without C2 ceramide (20 μM) measured by qRT-PCR.

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Biological Activity
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Description

IC₅₀ & Target

Protein phosphatase 1^[2]
PP2A^[4]
Ceramide-activated protein phosphatase (CAPP)^[4]
Apoptosis^[1]
Mitochondrial respiratory chain complex III^[1]

Cellular Effect

Cell Line	Type	Value	Description	References
CCRF-CEM	IC₅₀	31.6 μM Compound: C2-ceramide	In vitro growth inhibitory effect in human T-cell acute leukemia cells CCRF-CEM In vitro growth inhibitory effect in human T-cell acute leukemia cells CCRF-CEM	[PMID: 11689086]
FL5.12	IC₅₀	33 μM Compound: 9	Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis Cytotoxicity against mouse FL5.12A cells after 48 hrs by DAPI staining-based flow cytometric analysis	[PMID: 27475534]
HCT-116	EC₅₀	24.5 μM Compound: 3, C2-ceramide	Cytotoxicity against HCT116 cells assessed as cell survival by SRB assay Cytotoxicity against HCT116 cells assessed as cell survival by SRB assay	[PMID: 17561396]
HL-60	IC₅₀	26 μM Compound: 1a	Antiproliferative activity against human HL60 cells after 24 hrs by Alamar blue assay Antiproliferative activity against human HL60 cells after 24 hrs by Alamar blue assay	[PMID: 25172146]
HT-29	IC₅₀	25.9 μM Compound: C2-ceramide	Antiproliferative activity against human HT-29 cells measured after 72 hrs by sulforhodamine B assay Antiproliferative activity against human HT-29 cells measured after 72 hrs by sulforhodamine B assay	[PMID: 33592537]
HepG2	IC₅₀	58 μM Compound: 1a	Antiproliferative activity against human HepG2 cells after 24 hrs by Alamar blue assay Antiproliferative activity against human HepG2 cells after 24 hrs by Alamar blue assay	[PMID: 25172146]
K562	EC₅₀	35.1 μM Compound: 3, C2-ceramide	Cytotoxicity against K562 cells assessed as cell survival by MTT assay Cytotoxicity against K562 cells assessed as cell survival by MTT assay	[PMID: 17561396]
MCF7	IC₅₀	45.41 μM Compound: 1, C2 ceramide	Cytotoxicity against human MCF7 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human MCF7 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]
MDA-MB-231	IC₅₀	35.37 μM Compound: 1, C2 ceramide	Cytotoxicity against human MDA-MB-231 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human MDA-MB-231 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]
NCI-H358	EC₅₀	19.6 μM Compound: 3, C2-ceramide	Cytotoxicity against NCI-H358 cells assessed as cell survival by SRB assay Cytotoxicity against NCI-H358 cells assessed as cell survival by SRB assay	[PMID: 17561396]
NHDF	IC₅₀	66.5 μM Compound: 1, C2 ceramide	Cytotoxicity against NHDF after 24 hrs by CellTiter-Blue assay Cytotoxicity against NHDF after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]
PC-12	IC₅₀	98 μM Compound: 1a	Antiproliferative activity against rat PC12 cells after 24 hrs by Alamar blue assay Antiproliferative activity against rat PC12 cells after 24 hrs by Alamar blue assay	[PMID: 25172146]
SK-BR-3	IC₅₀	49.2 μM Compound: 1, C2 ceramide	Cytotoxicity against human SKBR3 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human SKBR3 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]
SUP-T1	IC₅₀	41.44 μM Compound: 1, C2 ceramide	Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]
SUP-T1	IC₅₀	> 200 μM Compound: 2	Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay Cytotoxicity against human SupT1 cells after 24 hrs by CellTiter-Blue assay	[PMID: 19171486]

In Vitro

C2 Ceramide (5 nM-200 μM; 24 hours; primary mouse osteoblasts) treatment (≤500 nM) promots osteoblast viability, whilst concentrations ≥2 μM significantly reduces osteoblast viability in a dose- and time-dependent manner^[1].
C2 Ceramide increases cytoplasmic histone-associated DNA fragments by 5.7- and 11.2-fold at 50 μM and 100 μM C2 Ceramide concentrations respectively in osteoblasts. At these higher concentrations, C2 Ceramide is a potent inducer of apoptosis in osteoblasts^[1].
C2 Ceramide up-regulates mRNA expression of angiogenic genes in human dental pulp cells (HDPCs) and increases the migration and capillary tube formation of endothelial cells, whereas PP1 small interfering RNA shows opposite effects. Human dental pulp cells (HDPCs) increases levels of bone morphogenetic protein 2, phosphorylation of Smad 1/5/8, and mRNA expression of runt-related transcription factor 2 and osterix^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:	Primary mouse osteoblasts
Concentration:	5 nM-200 µM
Incubation Time:	24 hours
Result:	Murine osteoblasts demonstrated a dose-dependent increase in their survival rate when exposed to low concentrations of 5-500 n M. Increasing concentrations of 20-200µM caused a dose-dependent decrease in mitochondrial succinate dehydrogenase activity and osteoblast survival.

In Vivo

The PP1 activator C2 Ceramide increases alkaline phosphatase activity, mineralizes nodule formation, and mRNA expression of dentin matrix protein 1 and dentin sialophosphoprotein. In contrast, knockdown by PP1 small interfering RNA inhibits odontoblastic differentiation^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

341.53

Formula

C₂₀H₃₉NO₃

CAS No.

3102-57-6

Appearance

Solid

Color

White to off-white

SMILES

O[C@H](/C=C/CCCCCCCCCCCCC)[C@H](CO)NC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

Ethanol : 100 mg/mL (292.80 mM; Need ultrasonic)

DMSO : 50 mg/mL (146.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9280 mL	14.6400 mL	29.2800 mL
5 mM	0.5856 mL	2.9280 mL	5.8560 mL
10 mM	0.2928 mL	1.4640 mL	2.9280 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% EtOH 90% Corn Oil
Solubility: 5 mg/mL (14.64 mM); Clear solution; Need ultrasonic

This protocol yields a clear solution of 5 mg/mL. If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL EtOH stock solution (50.0 mg/mL) to 900 μL Corn oil, and mix evenly.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (7.32 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.85%

Select Batch:

Data Sheet (273 KB) SDS (252 KB)

COA (240 KB) HNMR (279 KB) RP-HPLC (241 KB) MS (70 KB)

Handling Instructions (2659 KB)

References

[1]. P A Hill, et al. Ceramide-induced Cell Death/Survival in Murine Osteoblasts. J Endocrinol. 2010 Aug;206(2):225-33. [Content Brief]

[2]. Ji-Youn Kim, et al. Role of Protein Phosphatase 1 in Angiogenesis and Odontoblastic Differentiation of Human Dental Pulp Cells. J Endod. 2017 Mar;43(3):417-424. [Content Brief]

[3]. R T Dobrowsky, et al. Ceramide Stimulates a Cytosolic Protein Phosphatase. J Biol Chem. 1992 Mar 15;267(8):5048-51. [Content Brief]

[4]. R T Dobrowsky, et al. Ceramide Activates Heterotrimeric Protein Phosphatase 2A. J Biol Chem. 1993 Jul 25;268(21):15523-30. [Content Brief]

[5]. Maria Bernadete Riemma Pierre, et al. Influence of ceramide 2 on in vitro skin permeation and retention of 5-ALA and its ester derivatives, for Photodynamic Therapy. Brazilian Journal of Pharmaceutical Sciences. 2009, 45(1), 109-116.

[6]. Wenyuan Zhu, et al. C2-ceramide induces cell death and protective autophagy in head and neck squamous cell carcinoma cells. Int J Mol Sci. 2014 Feb 21;15(2):3336-55. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / Ethanol	1 mM	2.9280 mL	14.6400 mL	29.2800 mL	73.2000 mL
	5 mM	0.5856 mL	2.9280 mL	5.8560 mL	14.6400 mL
	10 mM	0.2928 mL	1.4640 mL	2.9280 mL	7.3200 mL
	15 mM	0.1952 mL	0.9760 mL	1.9520 mL	4.8800 mL
	20 mM	0.1464 mL	0.7320 mL	1.4640 mL	3.6600 mL
	25 mM	0.1171 mL	0.5856 mL	1.1712 mL	2.9280 mL
	30 mM	0.0976 mL	0.4880 mL	0.9760 mL	2.4400 mL
	40 mM	0.0732 mL	0.3660 mL	0.7320 mL	1.8300 mL
	50 mM	0.0586 mL	0.2928 mL	0.5856 mL	1.4640 mL
	60 mM	0.0488 mL	0.2440 mL	0.4880 mL	1.2200 mL
	80 mM	0.0366 mL	0.1830 mL	0.3660 mL	0.9150 mL
	100 mM	0.0293 mL	0.1464 mL	0.2928 mL	0.7320 mL

C2 Ceramide Related Classifications

Metabolic Disease Endocrinology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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C2 Ceramide (Synonyms: Ceramide 2)

Customer Review

Other Forms of C2 Ceramide:

C2 Ceramide Related Antibodies

2 Publications Citing Use of MCE C2 Ceramide

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References

Customer Review

C2 Ceramide Related Antibodies

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Complete Stock Solution Preparation Table

C2 Ceramide Related Classifications

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