Carboxy-SNARF 1 (5/6-mixture)  (Synonyms: SNARF 1)

Carboxy-SNARF 1 (5/6-mixture) (SNARF 1) is a fluorescent probe that is sensitive to pH (Ex: 488 nm). Carboxy-SNARF 1 (5/6-mixture) can be used for measurement pH. Carboxy-SNARF 1 (5/6-mixture) exhibits a significant emission shift from yellow-orange (Em: 580 nm) to deep red fluorescence (Em: 640 nm) under acidic and basic conditions (pH=7-8), respectively^[1].

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Carboxy-SNARF 1 (5/6-mixture) for fluorescent staining of liposomes^[1]
1. Preparation of working solution
Dissolve Carboxy-SNARF 1 (5/6-mixture) in HEPES/50 mM KCl buffer (pH7.5) to prepare a 0.1 mM Carboxy-SNARF 1 (5/6-mixture) working solution.
2. Specific staining steps
1) Weigh 10 mg of phospholipid, which can be dioleoyl L-phosphatidylcholine (DOPC) or egg yolk lecithin (EYL).
2) Dissolve the phospholipid in 0.5 mL chloroform and place it in an Eppendorf tube.
3) Dry the solution under a stream of nitrogen to form a lipid film on the inner wall of the tube.
4) Place the tube in a vacuum for 10 min.
5) Add 0.5 mL of 0.1 mM Carboxy-SNARF 1 (5/6-mixture) working solution to the vial containing the lipid film.
6) Mix the solution thoroughly using a vortex shaker to form multilamellar vesicles.
7) Freeze the liposome suspension in liquid nitrogen.
8) After thawing, prepare large unilamellar vesicles (LUVs) with a diameter of 400 nm by extrusion using a LipoFast extrusion device.
9) Remove the dye outside the liposomes by gel filtration. Centrifuge at 600 g for 5 min in pH 7.5 buffer using a 5 cm Sephadex G25 column.
10) The stained LUVs are ready for fluorescence measurement.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

453.44

C₂₇H₁₉NO₆

126208-12-6

Solid

530

488

O=C1C=C2C=CC3=C(C4=CC=C(C(O)=O)C=C4C(O)=O)C5=CC=C(N(C)C)C=C5OC3=C2C=C1.O=C6C=C7C=CC8=C(C9=CC(C(O)=O)=CC=C9C(O)=O)C%10=CC=C(N(C)C)C=C%10OC8=C7C=C6

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Vecer J, et, al. Fluorescence behavior of the pH-sensitive probe carboxy SNARF-1 in suspension of liposomes. Photochem Photobiol. 2001 Jul;74(1):8-13.  [Content Brief]