Casein Kinase II Inhibitor IV hydrochloride

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Casein Kinase II Inhibitor IV Hydrochloride is a small-molecule inducer of epidermal keratinocyte differentiation.

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Casein Kinase II Inhibitor IV hydrochloride 화학구조

CAS No. : 2320347-54-2

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50 mg		견적 받기
100 mg		견적 받기
250 mg		견적 받기
Other size		견적 받기

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Other In-stock Forms of Casein Kinase II Inhibitor IV hydrochloride:

Casein Kinase II Inhibitor IV

Other Forms of Casein Kinase II Inhibitor IV hydrochloride:

Casein Kinase II Inhibitor IV In-stock

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Biological Activity
Protocol
순도&문서
References
고객리뷰

제품 설명	Casein Kinase II Inhibitor IV Hydrochloride is a small-molecule inducer of epidermal keratinocyte differentiation.
IC₅₀ & Target	Target: Casein Kinase^[1]
In Vitro	Treatment of human epidermal keratinocytes (NHEKs) with Casein Kinase II Inhibitor IV leads to an increase in the early differentiation markers keratins 1 and 10 at 48 h. Increased levels of IVL and TGM are observed in cells treated with Casein Kinase II Inhibitor IV at 72 h and persisted at 96 h. In addition, treated with Casein Kinase II Inhibitor IV expresses loricrin, a terminal differentiation marker, at later time points. Similar results are observed by messenger RNA (mRNA) expression analysis of NHEKs treated with Casein Kinase II Inhibitor IV. At early time points (12 and 24 h), treatment with Casein Kinase II Inhibitor IV leads to the upregulation of keratinocyte early differentiation marker genes, including keratin 1 (5.4-fold) and keratin 10 (5.4-fold). Terminal differentiation marker genes, including IVL (1.8-fold), TGM 1 (4.8-fold), loricrin (3.3-fold), and filaggrin (5.6-fold), are upregulated at late time points (36 and 48 h). These results are consistent with the ability of Casein Kinase II Inhibitor IV to induce differentiation of epidermal progenitor cells into terminally differentiated keratinocytes^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Casein Kinase II Inhibitor IV hydrochloride Related Antibodies
분자량	465.93
화학식	C₂₄H₂₄ClN₅O₃
CAS No.	2320347-54-2
SMILES	N#CCCC1=CC=CC(N2C=CC3=CN=C(NC4=CC(OC)=C(OC)C(OC)=C4)N=C32)=C1.Cl
선적	Room temperature in continental US; may vary elsewhere.
보관	Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서	Data Sheet (271 KB) Handling Instructions (2659 KB)
References	[1]. Hong J, et al. Identification and characterization of small-molecule inducers of epidermal keratinocyte differentiation. ACS Chem Biol. 2007 Mar 20;2(3):171-5. [Content Brief]

Kinase Assay ^[1]	For reporter gene assays with transiently transfected cells, the cells are typically transfected in 150 mm-diam dishes when 30-40% confluent. A reporter plasmid, pGL3/3.7 kbp-IVLLuc plasmid, is transfected into the human epidermal keratinocytes (NHEKs). After 24 h, the transfected cells are plated into 96-well assay plates and treated with Casein Kinase II Inhibitor IV to a final concentration of 5 μM. After incubation for 2 d, reporter gene activity is measured using the Bright-Glo luciferase assay system^[1]. MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.
References	[1]. Hong J, et al. Identification and characterization of small-molecule inducers of epidermal keratinocyte differentiation. ACS Chem Biol. 2007 Mar 20;2(3):171-5. [Content Brief]

Kinase Assay
^[1]

For reporter gene assays with transiently transfected cells, the cells are typically transfected in 150 mm-diam dishes when 30-40% confluent. A reporter plasmid, pGL3/3.7 kbp-IVLLuc plasmid, is transfected into the human epidermal keratinocytes (NHEKs). After 24 h, the transfected cells are plated into 96-well assay plates and treated with Casein Kinase II Inhibitor IV to a final concentration of 5 μM. After incubation for 2 d, reporter gene activity is measured using the Bright-Glo luciferase assay system^[1].

MCE 는 독립적으로 이러한 방법들의 정확성을 확인하지 않았습니다. 참고용으로만 봐주십시오.

References

[1]. Hong J, et al. Identification and characterization of small-molecule inducers of epidermal keratinocyte differentiation. ACS Chem Biol. 2007 Mar 20;2(3):171-5. [Content Brief]