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  3. Cy5.5-COOH

Cy5.5-COOH  (Synonyms: Cyanine 5.5 carboxylic acid)

Art. -Nr.: HY-D1040 Reinheit: 98.82%
Handling Instructions Technical Support

Cy5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye that emits fluorescence at 710 nm upon excitation at 650 nm. Cy5.5-COOH possesses excellent spectral properties, including a narrow absorption spectrum, high sensitivity, and stability. Cy5.5-COOH can be used for bioimaging and disease diagnosis.

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Cy5.5-COOH

Cy5.5-COOH Chemische Struktur

CAS. Nr. : 1449612-07-0

Größe Preis Verfügbarkeit Menge
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
Auf Lager
Solution
10 mM * 1 mL in DMSO Auf Lager
Solid
5 mg Auf Lager
10 mg Auf Lager
25 mg Auf Lager
50 mg   Angebot einholen  
100 mg   Angebot einholen  

* Bitte wählen Sie Menge, bevor Sie Artikel hinzufügen.

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Beschreibung

Cy5.5-COOH (Cyanine 5.5 carboxylic acid) is a fluorescent dye that emits fluorescence at 710 nm upon excitation at 650 nm. Cy5.5-COOH possesses excellent spectral properties, including a narrow absorption spectrum, high sensitivity, and stability. Cy5.5-COOH can be used for bioimaging and disease diagnosis[1].

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Brief steps for labeling polypropylene (PP) microplastics with Cy5.5-COOH for bioimaging[1]:
1. Labeling PP microplastics: Mix PP microplastics with tetrahydrofuran and distilled water, add Cy5.5-COOH solution in DMSO, stir for 4 days, then filter, wash, and dry.
2. Verifying labeling efficiency: Confirm particle size and morphology via SEM and PSA, validate chemical structure by FT-IR, and detect fluorescence intensity using IVIS imaging.
3. Stability tests: Evaluate fluorescence stability in corn oil and digestive stability in simulated gastrointestinal fluids.
4. Biodistribution study: After oral administration of Cy-PP to mice, monitor real-time in vivo distribution at different time points via IVIS, and detect fluorescence signals in organs and feces at 24 hours.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molekulargewicht

582.77

Formel

C40H42N2O2

CAS. Nr.
Appearance

Solid

Color

Brown to reddish brown

SMILES

C[N+]1=C(/C=C/C=C/C=C2N(CCCCCC([O-])=O)C3=C(C4=CC=CC=C4C=C3)C/2(C)C)C(C)(C)C5=C1C=CC6=CC=CC=C56

Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : 50 mg/mL (85.80 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.7159 mL 8.5797 mL 17.1594 mL
5 mM 0.3432 mL 1.7159 mL 3.4319 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.7159 mL 8.5797 mL 17.1594 mL 42.8986 mL
5 mM 0.3432 mL 1.7159 mL 3.4319 mL 8.5797 mL
10 mM 0.1716 mL 0.8580 mL 1.7159 mL 4.2899 mL
15 mM 0.1144 mL 0.5720 mL 1.1440 mL 2.8599 mL
20 mM 0.0858 mL 0.4290 mL 0.8580 mL 2.1449 mL
25 mM 0.0686 mL 0.3432 mL 0.6864 mL 1.7159 mL
30 mM 0.0572 mL 0.2860 mL 0.5720 mL 1.4300 mL
40 mM 0.0429 mL 0.2145 mL 0.4290 mL 1.0725 mL
50 mM 0.0343 mL 0.1716 mL 0.3432 mL 0.8580 mL
60 mM 0.0286 mL 0.1430 mL 0.2860 mL 0.7150 mL
80 mM 0.0214 mL 0.1072 mL 0.2145 mL 0.5362 mL
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Cy5.5-COOH
Art. -Nr.:
HY-D1040
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