Neurotrophin-3 Protein, Human

Neurotrophin-3 Protein, Human is a recombinant Neurotrophin-3 protein expressed in E. coli system. Neurotrophin-3 is widely expressed in the nervous system. Neurotrophin-3 reduces cellular damage, improves neuronal regeneration in different models of lesions^[1].

Neurotrophin-3 (NT-3), a trophic factor from the neurotrophin family, is widely expressed in the nervous system.
The physiological actions of NT-3 are mediated by the activation of two membrane receptors: the low-affinity receptor p75 and the high-affinity receptor Trk. NT-3 activates the TrkC receptor and binds with less affinity to TrkB and TrkA.
NT-3 reduces cellular damage, improves neuronal regeneration in different models of lesions or neurodegeneration, and participates in synaptic reorganization, synapse formation, and neuronal plasticity^[1].

Neurotrophin-3 (human; 2 ng/mL; 20 h), but not brain-derived neurotrophic factor, promotes neuronal differentiation of retinal progenitor E4 cells^[3].

1.The ED₅₀ as determined by its ability to bind Human NTRK2 in functional ELISA is less than 10 μg/mL.
2.Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 33.69-35.88 ng/mL, corresponding to a specific activity is 2.787-2.968×10⁴ units/mg.

• 
  Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 35.88 ng/mL, corresponding to a specific activity is 2.787×10⁴ units/mg.

Human

E. coli

Tag Free

P20783-1 (Y139-T257)

4908  [NCBI]

Full Length of Isoform-1 Mature Protein

YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEARPVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT

Approximately 14-15 kDa, based on SDS-PAGE under reducing conditions.

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder

1.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.
2.Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 20% Acetonitrile, pH 2.5.
Please refer to the lot-specific COA for specific buffer information.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Koichi Uegaki, et al. BDNF Binds Its Pro-Peptide with High Affinity and the Common Val66 Met Polymorphism Attenuates the Interaction. Int J Mol Sci. 2017 May 12;18(5):1042.  [Content Brief]

  [2]. O Blondel, et al. A glia-derived signal regulating neuronal differentiation. J Neurosci. 2000 Nov 1;20(21):8012-20.  [Content Brief]

  [3]. de la Rosa EJ, et al. Role of neurotrophins in the control of neural development: neurotrophin-3 promotes both neuron differentiation and survival of cultured chick retinal cells. Neuroscience. 1994 Jan;58(2):347-52.  [Content Brief]