2. Apoptosis
  3. Apoptosis
  4. Didymin

Didymin, a flavonoid glycoside, possesses antioxidant and anticancer properties. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma.

For research use only. We do not sell to patients.

CAS No. : 14259-47-3

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Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Didymin:

Didymin Related Antibodies

Top Publications Citing Use of Products

    Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604.  [Abstract]

    TRAP staining to detect osteoclastogenesis of PBMCs with various concentrations of Didymin (10, 20 μM) treatment.

    Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604.  [Abstract]

    The mRNA level of ACP5, CTSK, NFATc1 in PBMCs with vehicle or Didymin (10, 20 μM) treatment in the presence or absence of h-RANKL for 10 days.

    Didymin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Aug 15;16(1):7604.  [Abstract]

    Western blots of RKIP, c-Fos, and NFATc1 protein levels in PBMCs with vehicle or Didymin (10, 20 μM) treatment in the presence or absence of h-RANKL for 10 days.

    Didymin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Dec 19;21(1):921.  [Abstract]

    Cell viability of AML12 cells treated with PA and different concentrations of Didymin. (10, 20, 50 μM).

    Didymin purchased from MedChemExpress. Usage Cited in: J Transl Med. 2023 Dec 19;21(1):921.  [Abstract]

    Co-treatment of Didymin (50 μM) and EX-527 resulted in a re-increase in the number of apoptotic cells by Flow cytometry analysis.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Didymin, a flavonoid glycoside, possesses antioxidant and anticancer properties. Didymin induces apoptosis by inhibiting N-Myc and upregulating RKIP in neuroblastoma[1].

    In Vitro

    Didymin (1-20 μM; 48 h) inhibits cell proliferation in human non-small-cell lung cancer cells[1].
    Didymin (10-20 μM; 12-48 h) induced apoptosis in A549 and H460 cells, without affecting the cell cycle distribution.[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Didymin Related Antibodies

    Cell Proliferation Assay[1]

    Cell Line: A549 and H460 cells
    Concentration: 1 μM, 5 μM, 10 μM, 20 μM
    Incubation Time: 48 h
    Result: Inhibited cell proliferation in human non-small-cell lung cancer cells.

    Apoptosis Analysis[1]

    Cell Line: A549 and H460 cells
    Concentration: 10 μM, 20 μM
    Incubation Time: 12 h, 24 h, 48 h
    Result: Induced apoptosis in A549 and H460 cells, without affecting the cell cycle distribution.
    In Vivo

    Didymin (6 mg/kg/day; i.p.; daily for 28 days) delays the tumor growth in nude mice[1].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Male nude mice [6 weeks old; BALB/cA-nu (nu/nu)] injected with A549 cells[1]
    Dosage: 6 mg/kg/day
    Administration: i.p.; daily for 28 days
    Result: Inhibited tumor growth in nude mice.
    Molecular Weight

    594.56

    Formula

    C28H34O14
    CAS No.
    Appearance

    Solid

    Color

    White to yellow

    SMILES

    O=C1C[C@@H](C2=CC=C(OC)C=C2)OC3=CC(O[C@H]4[C@@H]([C@H]([C@@H]([C@@H](CO[C@H]5[C@@H]([C@@H]([C@H]([C@H](C)O5)O)O)O)O4)O)O)O)=CC(O)=C13
    Structure Classification
    Initial Source
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 250 mg/mL (420.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.6819 mL 8.4096 mL 16.8192 mL
    5 mM 0.3364 mL 1.6819 mL 3.3638 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 2.17 mg/mL (3.65 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 2.17 mg/mL (3.65 mM); Clear solution

      This protocol yields a clear solution of ≥ 2.17 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (21.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.96%

    SDS (393 KB)

    COA (248 KB) LCMS (89 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.6819 mL 8.4096 mL 16.8192 mL 42.0479 mL
    5 mM 0.3364 mL 1.6819 mL 3.3638 mL 8.4096 mL
    10 mM 0.1682 mL 0.8410 mL 1.6819 mL 4.2048 mL
    15 mM 0.1121 mL 0.5606 mL 1.1213 mL 2.8032 mL
    20 mM 0.0841 mL 0.4205 mL 0.8410 mL 2.1024 mL
    25 mM 0.0673 mL 0.3364 mL 0.6728 mL 1.6819 mL
    30 mM 0.0561 mL 0.2803 mL 0.5606 mL 1.4016 mL
    40 mM 0.0420 mL 0.2102 mL 0.4205 mL 1.0512 mL
    50 mM 0.0336 mL 0.1682 mL 0.3364 mL 0.8410 mL
    60 mM 0.0280 mL 0.1402 mL 0.2803 mL 0.7008 mL
    80 mM 0.0210 mL 0.1051 mL 0.2102 mL 0.5256 mL
    100 mM 0.0168 mL 0.0841 mL 0.1682 mL 0.4205 mL
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    Didymin Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Didymin14259-47-3ApoptosisInhibitorinhibitorinhibit

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