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  4. Disuccinimidyl sulfoxide

Disuccinimidyl sulfoxide  (Synonyms: DSSO)

製品番号: HY-123740 純度: 99.83%
COA 取扱説明書 Technical Support

Disuccinimidyl sulfoxide (DSSO) is a sulfoxide-containing crosslinker targeting amino groups that can be cleaved by MS, and it is suitable for model peptides, proteins, and multisubunit protein complexes. Disuccinimidyl sulfoxide contains two symmetric collision-induced dissociation (CID)-cleavable sites, enabling the identification of DSSO-crosslinked peptides based on different fragmentation patterns.

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Disuccinimidyl sulfoxide

Disuccinimidyl sulfoxide 構造式

CAS 番号 : 1351828-03-9

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
10 mg $33 在庫あり
25 mg $65 在庫あり
50 mg $110 在庫あり
100 mg $190 在庫あり
250 mg $380 在庫あり
500 mg $600 在庫あり
1 g $900 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Disuccinimidyl sulfoxide 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

  • 参考文献

  • カスタマーレビュー

製品説明

Disuccinimidyl sulfoxide (DSSO) is a sulfoxide-containing crosslinker targeting amino groups that can be cleaved by MS, and it is suitable for model peptides, proteins, and multisubunit protein complexes. Disuccinimidyl sulfoxide contains two symmetric collision-induced dissociation (CID)-cleavable sites, enabling the identification of DSSO-crosslinked peptides based on different fragmentation patterns[1].

体外実験

Disuccinimidyl sulfoxide contains two symmetric collision-induced dissociation (CID)-cleavable sites, which enables the identification of DSSO-crosslinked peptides based on different cleavage patterns[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Disuccinimidyl sulfoxide 関連抗体
分子量

388.35

分子式

C14H16N2O9S
CAS 番号
Appearance

Solid

Color

White to off-white

SMILES

O=C(ON1C(CCC1=O)=O)CCS(CCC(ON2C(CCC2=O)=O)=O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
溶剤 & 溶解度
体外: 

DMSO : 20 mg/mL (51.50 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

DMF : 10 mg/mL (25.75 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.5750 mL 12.8750 mL 25.7500 mL
5 mM 0.5150 mL 2.5750 mL 5.1500 mL
10 mM 0.2575 mL 1.2875 mL 2.5750 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: 99.83%

COA (248 KB) RP-HPLC (246 KB) MS (69 KB)

取扱説明書 (2659 KB)
参考文献

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMF / DMSO 1 mM 2.5750 mL 12.8750 mL 25.7500 mL 64.3749 mL
5 mM 0.5150 mL 2.5750 mL 5.1500 mL 12.8750 mL
10 mM 0.2575 mL 1.2875 mL 2.5750 mL 6.4375 mL
15 mM 0.1717 mL 0.8583 mL 1.7167 mL 4.2917 mL
20 mM 0.1287 mL 0.6437 mL 1.2875 mL 3.2187 mL
25 mM 0.1030 mL 0.5150 mL 1.0300 mL 2.5750 mL
DMSO 30 mM 0.0858 mL 0.4292 mL 0.8583 mL 2.1458 mL
40 mM 0.0644 mL 0.3219 mL 0.6437 mL 1.6094 mL
50 mM 0.0515 mL 0.2575 mL 0.5150 mL 1.2875 mL
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Disuccinimidyl sulfoxide Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Disuccinimidyl sulfoxide1351828-03-9DSSOBiochemical Assay ReagentscrosslinkerInhibitorinhibitorinhibit

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製品名:
Disuccinimidyl sulfoxide
製品番号:
HY-123740
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