Disuccinimidyl suberate Crosslinker

Name: Disuccinimidyl suberate Crosslinker
Brand: MedChemExpress
SKU: HY-W019543
Rating: 5.0 (14 reviews)

Cat. No.: HY-W019543 Purity: 98.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

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Disuccinimidyl suberate Crosslinker 화학구조

CAS No. : 68528-80-3

사이즈	재고	수량
무료 샘플 (0.1 - 0.2 mg)	지금 신청하기
100 mg	해외재고보유	Estimated Time of Arrival: December 31
500 mg	해외재고보유	Estimated Time of Arrival: December 31
1 g	해외재고보유	Estimated Time of Arrival: December 31
5 g	견적 받기
10 g	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 14 publication(s) in Google Scholar

Other Forms of Disuccinimidyl suberate Crosslinker:

Disuccinimidyl suberate Crosslinker-d₄ 견적 받기

14 Publications Citing Use of MCE Disuccinimidyl suberate Crosslinker

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•Related Small Molecules:

•Related Proteins:

Biological Activity
순도&문서
고객리뷰

제품 설명

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

In Vitro

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. Disuccinimidyl suberate Crosslinker is first dissolved in an organic solvent such as DMSO, then added to the aqueous crosslinking reaction.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

분자량

368.34

화학식

C₁₆H₂₀N₂O₈

CAS No.

68528-80-3

Appearance

Solid

Color

White to light yellow

SMILES

O=C(ON1C(CCC1=O)=O)CCCCCCC(ON2C(CCC2=O)=O)=O

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (271.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.7149 mL	13.5744 mL	27.1488 mL
5 mM	0.5430 mL	2.7149 mL	5.4298 mL
10 mM	0.2715 mL	1.3574 mL	2.7149 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: ≥98.0%

Select Batch:

Data Sheet (246 KB) SDS (393 KB)

COA (247 KB) HNMR (161 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.7149 mL	13.5744 mL	27.1488 mL	67.8721 mL
	5 mM	0.5430 mL	2.7149 mL	5.4298 mL	13.5744 mL
	10 mM	0.2715 mL	1.3574 mL	2.7149 mL	6.7872 mL
	15 mM	0.1810 mL	0.9050 mL	1.8099 mL	4.5248 mL
	20 mM	0.1357 mL	0.6787 mL	1.3574 mL	3.3936 mL
	25 mM	0.1086 mL	0.5430 mL	1.0860 mL	2.7149 mL
	30 mM	0.0905 mL	0.4525 mL	0.9050 mL	2.2624 mL
	40 mM	0.0679 mL	0.3394 mL	0.6787 mL	1.6968 mL
	50 mM	0.0543 mL	0.2715 mL	0.5430 mL	1.3574 mL
	60 mM	0.0452 mL	0.2262 mL	0.4525 mL	1.1312 mL
	80 mM	0.0339 mL	0.1697 mL	0.3394 mL	0.8484 mL
	100 mM	0.0271 mL	0.1357 mL	0.2715 mL	0.6787 mL