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  4. Disuccinimidyl suberate Crosslinker

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

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Disuccinimidyl suberate Crosslinker

DSS Crosslinker 構造式

CAS 番号 : 68528-80-3

容量 価格（税別） 在庫状況 数量
>無料サンプル (0.1 - 0.2 mg)   今すぐ申し込む  
100 mg $50 在庫あり
500 mg $70 在庫あり
1 g $90 在庫あり
5 g   お問い合わせ  
10 g   お問い合わせ  

* アイテムを追加する前、数量をご選択ください

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Based on 14 publication(s) in Google Scholar

Other Forms of Disuccinimidyl suberate Crosslinker:

DSS Crosslinker 関連抗体

Top Publications Citing Use of Products

  • 生物活性

  • 純度とドキュメンテーション

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製品説明

Disuccinimidyl suberate Crosslinker is an amine-reactive bifunctional protein crosslinker. Disuccinimidyl suberate Crosslinker enables stable protein-protein, peptide, and biomolecule conjugation.

体外実験

NHS esters react with primary amines at pH 7-9 to form stable amide bonds, along with release of the N-hydroxysuccinimide leaving group. Proteins, including antibodies, generally have several primary amines in the side chain of lysine (K) residues and the N-terminus of each polypeptide that are available as targets for NHS-ester crosslinking reagents. Disuccinimidyl suberate Crosslinker is first dissolved in an organic solvent such as DMSO, then added to the aqueous crosslinking reaction.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

DSS Crosslinker 関連抗体
分子量

368.34

分子式

C16H20N2O8
CAS 番号
Appearance

Solid

Color

White to light yellow

SMILES

O=C(ON1C(CCC1=O)=O)CCCCCCC(ON2C(CCC2=O)=O)=O
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件
Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month
溶剤 & 溶解度
体外: 

DMSO : 100 mg/mL (271.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7149 mL 13.5744 mL 27.1488 mL
5 mM 0.5430 mL 2.7149 mL 5.4298 mL
10 mM 0.2715 mL 1.3574 mL 2.7149 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始）

C1

×
体積 （開始）

V1

=
濃度 （終了）

C2

×
体積 （終了）

V2

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    90% Corn Oil

    Solubility: ≥ 2.5 mg/mL (6.79 mM); Clear solution

    This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
% 
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
純度とドキュメンテーション

純度: ≥98.0%

COA (247 KB) HNMR (161 KB)

取扱説明書 (2659 KB)

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7149 mL 13.5744 mL 27.1488 mL 67.8721 mL
5 mM 0.5430 mL 2.7149 mL 5.4298 mL 13.5744 mL
10 mM 0.2715 mL 1.3574 mL 2.7149 mL 6.7872 mL
15 mM 0.1810 mL 0.9050 mL 1.8099 mL 4.5248 mL
20 mM 0.1357 mL 0.6787 mL 1.3574 mL 3.3936 mL
25 mM 0.1086 mL 0.5430 mL 1.0860 mL 2.7149 mL
30 mM 0.0905 mL 0.4525 mL 0.9050 mL 2.2624 mL
40 mM 0.0679 mL 0.3394 mL 0.6787 mL 1.6968 mL
50 mM 0.0543 mL 0.2715 mL 0.5430 mL 1.3574 mL
60 mM 0.0452 mL 0.2262 mL 0.4525 mL 1.1312 mL
80 mM 0.0339 mL 0.1697 mL 0.3394 mL 0.8484 mL
100 mM 0.0271 mL 0.1357 mL 0.2715 mL 0.6787 mL
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Disuccinimidyl suberate Crosslinker Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

質量   濃度   体積   分子量 *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

一般には略語で表示されます：C1V1 = C2V2

濃度 （開始） × 体積 （開始） = 濃度 （終了） × 体積 （終了）
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Disuccinimidyl suberate Crosslinker68528-80-3Biochemical Assay ReagentsInhibitorinhibitorinhibit

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製品名:
Disuccinimidyl suberate Crosslinker
製品番号:
HY-W019543
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