eGFP mRNA (N1-Me-Pseudo UTP)

製品番号: HY-153079 純度: 97.00%: Data Sheet SDS COA 取扱説明書
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eGFP mRNA (N1-Me-Pseudo UTP) will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence. The substitution with N1-Me-Pseudo UTP (m1Ψ) improves protein expression and reduces innate immune response.

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eGFP mRNA (N1-Me-Pseudo UTP) 構造式

容量	価格（税別）	在庫状況	数量
Solvent
100 μg (1 mg/mL * 100 μL in Water)	USD 320	在庫あり	Estimated Time of Arrival: December 31
Solvent
500 μg (1 mg/mL * 500 μL in Water)	USD 750	在庫あり	Estimated Time of Arrival: December 31
Solvent
1 mg (1 mg/mL * 1 mL in Water)	USD 1200	在庫あり	Estimated Time of Arrival: December 31

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製品説明	eGFP mRNA (N1-Me-Pseudo UTP) will express green fluorescent protein, originally isolated from the jellyfish, Aequorea victoria. The eGFP is a commonly used direct detection reporter in mammalian cell culture, yielding bright green fluorescence. The substitution with N1-Me-Pseudo UTP (m1Ψ) improves protein expression and reduces innate immune response.
体外実験	Transfected Hep 3B cells with unmodified, me1 ψ-, 5 moU-, or ψ-modified eGFP mRNAs. After 1h treatment, total RNA is collected at time intervals (t = 0, 1.5, 3, and 4.5 h) and reversely transcribed into complementary DNA (cDNA). The amount of each eGFP mRNA is calculated by quantitative PCR (qPCR) analysis in normalization of endogenous 18S rRNA^[1]. MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. eGFP mRNA (N1-Me-Pseudo UTP) 関連抗体
Appearance	Liquid
Color	Colorless to light yellow
SMILES	[eGFP mRNA (N1-Me-Pseudo UTP)]
輸送条件	Shipping with dry ice.
保管条件	-80°C
純度とドキュメンテーション	純度: 97.00% Select Batch: データシート (263 KB) SDS (251 KB) COA (232 KB) 取扱説明書 (2659 KB)
参考文献	[1]. Li B, Luo X, Dong Y. Effects of Chemically Modified Messenger RNA on Protein Expression. Bioconjug Chem. 2016;27(3):849-853. [Content Brief]