Firefly Luciferase mRNA

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Firefly Luciferase mRNA is a reporter mRNA that can be transfected into cells to express firefly luciferase protein. Firefly Luciferase mRNA induces cytotoxicity in cancer cells at low concentrations. In cancer cells, the expression level of luciferase shows a non-linear relationship with the dose of Firefly Luciferase mRNA. When combined with the H₂S-responsive bioluminescent probe (H-Luc), Firefly Luciferase mRNA enables bioluminescence-based detection of endogenous hydrogen sulfide in non-transgenic NAFLD cell models and NAFLD mouse models. Firefly Luciferase mRNA can be used in studies related to non-alcoholic fatty liver disease.

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Firefly Luciferase mRNA Estructura química

Tamaño	Precio	Stock	Cantidad
100 μg		En stock	Estimated Time of Arrival: December 31

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In Vitro

Firefly Luciferase mRNA (0.02-4.60 μg/mL; 5 h treatment incubation, 48 h post-treatment measurement) transfection of Jurkat cells results in limited, non-linear luciferase expression and significant cytotoxicity, with cell viability dropping to 40-50% at high mRNA concentrations^[1].
Firefly Luciferase mRNA (0.02-4.60 μg/mL; 5 h treatment incubation, 48 h post-treatment measurement) transfection of L-929 cells produces limited luciferase expression that peaks at 3 μg/mL mRNA before declining, with no associated cytotoxicity across tested concentrations^[1].
Firefly Luciferase mRNA (0.02-4.60 μg/mL; 5 h treatment incubation, 48 h post-treatment measurement) transfection of HEK 293 T cells produces a strong, linear dose-dependent increase in luciferase expression across 0.02 to 4.60 μg/mL mRNA with no cytotoxicity, but exhibits high intra-group variability^[1].
Firefly Luciferase mRNA (50 ng; 24 h) delivered via 4A3-SCC-PH LNPs to nontransfected MDA-MB-231 cells successfully produces functional firefly luciferase, enabling sensitive detection of endogenous H₂S via H-Luc with a 68-fold increase in bioluminescence intensity relative to untreated cells^[2].
Firefly Luciferase mRNA (50 ng; 24 h) delivered via 4A3-SCC-PH LNPs to nontransfected IGROV-1 cells successfully produces functional firefly luciferase, enabling sensitive detection of endogenous H₂S via H-Luc^[2].
Firefly Luciferase mRNA (50 ng; 24 h) delivered via 4A3-SCC-PH LNPs to oleic acid-induced NAFLD AML-12 cells successfully produces functional firefly luciferase, enabling detection of elevated endogenous H₂S with a 3.7-fold higher bioluminescence intensity relative to normal AML-12 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Firefly Luciferase mRNA-loaded 4A3-SCC-PH LNPs (0.1 mg/kg; i.v.; single dose) enables bioluminescence imaging of endogenous H₂S in NAFLD mouse livers, with NAFLD mice showing 28-fold higher liver bioluminescence intensity than normal mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 (female, 6-8 weeks old, NAFLD induced by 60% fat high-fat diet and intraperitoneal injection of dexamethasone sodium phosphate at 100 mg/kg every 2 days for 7 days)^[2]
Dosage:	0.1 mg/kg
Administration:	i.v.; single dose
Result:	Exhibited 28-fold higher bioluminescence intensity in the liver compared to normal mice. Detected bioluminescence signals within the first 5 minutes after H-Luc injection, reached maximum intensity at 10 minutes, and remained stable for at least 30 minutes. Showed intense bioluminescence signals in the liver and spleen via ex vivo imaging. Had liver bioluminescence signals suppressed by 19-fold after pretreatment with AOAA.

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Colorless to light yellow

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Purity: 90%

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Referencias

[1]. Zixuan Zhen, et al. Impact of cell line and reporter gene selection on in-vitro transfection evaluation of mRNA lipid nanoparticles. Zhen et al. AAPS Open. Volume 11, article number 20, (2025).

[2]. Chen Z, et al. Diagnosis of Nonalcoholic Fatty Liver Disease via a H₂S-Responsive Bioluminescent Probe Combined with Firefly Luciferase mRNA Delivery. Anal Chem. 2024;96(22):9236-9243.