Fluorol Yellow 088  (Synonyms: 2,8-Dimethylnaphtho[3,2,1-kl]xanthene)

Fluorol Yellow 088 is a fluorescent dye that can be used as a biological material or an organic compound for life science research. Fluorol Yellow 088 is suitable for lipid staining in plant tissues, particularly for the fluorescence imaging of suberized cell walls and hydrophobic structures, with an excitation wavelength of 365 nm and an emission wavelength of ≥420 nm^[1][2].

Guide (The following is our recommended protocol. This protocol is provided as a guide only and should be modified to suit your specific needs).
Lipid Staining in Plant Tissue^[1]
Fluorol Yellow 088 is a highly efficient fluorescent lipid dye suitable for lipid staining in plant tissues, particularly for the fluorescence imaging of suberized cell walls and hydrophobic structures.
I. Reagent Preparation
Staining solution:
(1) Take an appropriate amount of Fluorol Yellow 088, and prepare a 0.01% (w/v) solution using anhydrous DMSO.
(2) Dissolve the dye in polyethylene glycol (PEG 400) and heat at 90°C for 1 hour to ensure complete dissolution.
(3) Add an equal volume of 90% glycerol solution (containing 10% distilled water) and mix thoroughly.
II. Staining Procedure
Sample fixation:
(1) Use fresh tissue or 50% ethanol-fixed tissue for staining.
(2) Prepare thin sections using a razor blade or a microtome to ensure section integrity.
Sample staining:
(1) Immerse tissue sections in Fluorol Yellow 088 staining solution and incubate at room temperature for 1 hour.
(2) Lightly rinse the tissue to remove excess dye (brief rinsing is recommended to avoid excessive removal of the stain).
Mounting:
Use 75% glycerol as the mounting medium (to prevent fading).
III. Observation and Analysis
Fluorescence microscopy observation: excitation wavelength 365 nm, emission wavelength ≥ 420 nm.
Imaging can be recorded using Nomarski interference-contrast or fluorescence microscopy.
IV. Application Scope
Primarily used for lipid staining in plant tissues, especially for the visualization of suberin lamellae (Suberin lamellae).
Effectively stains endodermis (Endodermis) and exodermis (Exodermis).
Advantages:
Strong staining stability, not prone to precipitation in aqueous media.
High lipid specificity, providing high-contrast imaging.
Suitable for large-scale scanning (rapid screening under low magnification).

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

296.37

C₂₂H₁₆O

453

81-37-8

Solid

Brown to reddish brown

CC1=C2C3=C(C=C1)OC4=C(C=C(C)C=C4)C3=C5C=CC=CC5=C2

Room temperature in continental US; may vary elsewhere.

4°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

• [1]. Brundrett MC, et al. Efficient lipid staining in plant material with sudan red 7B or fluorol [correction of fluoral] yellow 088 in polyethylene glycol-glycerol. Biotech Histochem. 1991;66(3):111-6.  [Content Brief]

  [2]. Cheli F, et al. Fluorescent Labeling of Lignin Nanocapsules with Fluorol Yellow 088. Methods Mol Biol. 2023;2566:345-353.  [Content Brief]