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  3. Fluorescent Dye
  4. FM1-84

FM1-84 (Neurodye GH1-84) is a fluorescent dye. FM1-84 has lipophilic and facilitates association with membranes, resulting in an increase in fluorescence intensity (λex=510 nm, λem=625 nm). FM1-84 can be used for synaptic vesicle recycling in neurons research.

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FM1-84

FM1-84 Chemische Struktur

CAS. Nr. : 303727-66-4

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FM1-84 Related Antibodies

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Beschreibung

FM1-84 (Neurodye GH1-84) is a fluorescent dye. FM1-84 has lipophilic and facilitates association with membranes, resulting in an increase in fluorescence intensity (λex=510 nm, λem=625 nm). FM1-84 can be used for synaptic vesicle recycling in neurons research[1].

Molekulargewicht

639.59

Formel

C32H53Br2N3
CAS. Nr.
Appearance

Oil

Color

Brown to reddish brown

Emission (Em)

625
Excitation (Ex)

510
SMILES

CC[N+](CC)(CC)CCC[N+](C=C1)=CC=C1/C=C/C2=CC=C(N(CCCCC)CCCCC)C=C2.[Br-].[Br-]
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Lösungsmittel & Löslichkeit
In Vitro: 

DMSO : ≥ 125 mg/mL (195.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.5635 mL 7.8175 mL 15.6350 mL
5 mM 0.3127 mL 1.5635 mL 3.1270 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molaritätsrechner

  • Verdünnungsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Reinheit & Dokumentation
Verweise

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.5635 mL 7.8175 mL 15.6350 mL 39.0875 mL
5 mM 0.3127 mL 1.5635 mL 3.1270 mL 7.8175 mL
10 mM 0.1564 mL 0.7818 mL 1.5635 mL 3.9088 mL
15 mM 0.1042 mL 0.5212 mL 1.0423 mL 2.6058 mL
20 mM 0.0782 mL 0.3909 mL 0.7818 mL 1.9544 mL
25 mM 0.0625 mL 0.3127 mL 0.6254 mL 1.5635 mL
30 mM 0.0521 mL 0.2606 mL 0.5212 mL 1.3029 mL
40 mM 0.0391 mL 0.1954 mL 0.3909 mL 0.9772 mL
50 mM 0.0313 mL 0.1564 mL 0.3127 mL 0.7818 mL
60 mM 0.0261 mL 0.1303 mL 0.2606 mL 0.6515 mL
80 mM 0.0195 mL 0.0977 mL 0.1954 mL 0.4886 mL
100 mM 0.0156 mL 0.0782 mL 0.1564 mL 0.3909 mL
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FM1-84 Related Classifications

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

FM1-84303727-66-4Neurodye GH1-84Fluorescent Dyefluorescent dyecell Membranesynaptic vesicle recyclingInhibitorinhibitorinhibit

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