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  3. Protein Biology
  4. Protein Sample Preparation
  5. Protease Inhibitor Cocktail
  6. Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)

Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)  

Cat. No.: HY-K0011
Manual COA SDS Technical Support
Cocktails

MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity. The 25 pcs is defined as the base specification. All larger sizes correspond to incremental volumes of this base.

Tamaño Precio Stock Cantidad
Muestra gratis (2 pcs)   Solicite ahora  
25 pcs En stock
50 pcs En stock
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251 Publications Citing Use of MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)

WB

    Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) purchased from MCE. Usage Cited in: Mol Cell. 2024 May 29:S1097-2765(24)00434-9.  [Abstract]

    Expression of PABPC1 in H9 cells and FB neurons (A), H9 cells, and SH-SY5Y cells (B), revealed by western blotting. ACTIN was detected as control.

    • Descripciòn

    • Almacenamiento

    • Protocolo

    • Componentes

    • Documentación

    Description
    & Advantages

    Endogenous proteins are produced and removed in a balanced state, so their cellular levels are generally stable under stable environmental conditions. However, protein degradation is enhanced when cells are studied in vitro. To prevent the degradation of proteins under such conditions, one can utilize a cocktail of small molecule inhibitors to block the action of proteases.

    MCE Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) is a blend of 5 pan-protease inhibitors for protection of protein integrity. This product can be applied in Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, Kinase assay and etc.
    Almacenamiento

    4℃, 2 years
    Protocolo

    1. Direct Use: Add one tablet of the Protease Inhibitor Cocktail to 10 mL of the sample and mix thoroughly until dissolved.

    2. Preparation of Stock Solution: Dissolve one tablet of the Protease Inhibitor Cocktail in 1 mL of ddH2O, vortex to ensure complete dissolution, and prepare a 10× stock solution. Store at 4°C for up to one week or at -20°C for at least one month.
    Componentes
    Cat. No. Product Name Package
    HY-K0011-25 pcs Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) 25 pcs
    HY-K0011-50 pcs Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free) 25 pcs × 2
    Documentación

    Manual (1043 KB) SDS (4579 KB)
    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Protein Degradation InhibitorPhosphatase InhibitorProtein inhibitor

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    Nombre del producto:
    Protease Inhibitor Cocktail, mini-Tablet (EDTA-Free)
    Cat. No.:
    HY-K0011
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