2. Academic Validation
  3. Identification of lncRNA-Protein Interactions by CLIP and RNA Pull-Down Assays

Identification of lncRNA-Protein Interactions by CLIP and RNA Pull-Down Assays

  • Methods Mol Biol. 2021;2348:231-242. doi: 10.1007/978-1-0716-1581-2_16.
Kunming Zhao 1,  Xingwen Wang 1,  Ying Hu 2
Affiliations

Affiliations

  • 1 Harbin Institute of Technology, School of Life Sciences and Technology, Harbin, China.
  • 2 Harbin Institute of Technology, School of Life Sciences and Technology, Harbin, China. [email protected].
PMID: 34160811 DOI: 10.1007/978-1-0716-1581-2_16
Abstract

The emerging data indicates that long noncoding RNAs (lncRNAs) are involved in fundamental biological processes, and their deregulation may lead to oncogenesis and Other Diseases. LncRNA fulfil its biological functions at least in part by interacting with distinctive proteins. Here, we described two methods to identify the direct or indirect interactions between lncRNA and proteins: cross-linking and immunoprecipitation (CLIP) and RNA pull-down assay. CLIP methods enable yield a list of lncRNAs that directly interact target protein in living cells, whereas immunoprecipitation of biotin-labeled RNA (RNA pull-down) assay represents a method for identification of proteins that directly and indirectly bind with a particular target lncRNA of interest.

Keywords

CLIP; Immunoblots; Immunoprecipitation; RNA pull-down; UV cross-linking; lncRNA.

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