2. Others Cell Cycle/DNA Damage
  3. Biochemical Assay Reagents Eukaryotic Initiation Factor (eIF)
  4. ISRIB

ISRIB is a brain-penetrant inhibitor of integrated stress response (ISR). Persistent activation of the ISR has been linked to the development of several neurological disorders as ISR represses translation through inhibiting eIF2B. ISRIB inhibits the ISR by promoting the nucleotide exchange activity of eIF2B and recovering the translation, and thus can be used for neurological disorders research.

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CAS. Nr. : 548470-11-7

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Based on 26 publication(s) in Google Scholar

Other Forms of ISRIB:

ISRIB Related Antibodies

Top Publications Citing Use of Products

    ISRIB purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec:88:103905.  [Abstract]

    Schematic illustrating ISRIB treatment regimen (20 mg/kg, administered twice weekly from weeks 4–8) in CCl4-induced fibrotic mouse models. (B) Representative images of HE staining in liver sections after CCl4 treatment.

    ISRIB purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Dec:88:103905.  [Abstract]

    Schematic illustrating ISRIB treatment regimen (20 mg/kg, administered twice weekly from weeks 4–8) in CCl4-induced fibrotic mouse models. Relative gene expression levels of ATF4 in liver tissues after CCl4 injection for 8 weeks (n = 4).

    ISRIB purchased from MedChemExpress. Usage Cited in: Int J Oral Sci. 2024 May 8;16(1):35.  [Abstract]

    Protein expression levels of ATF4, DDIT4, NIBAN1, p62, ATG5, and LC3-I/II were analyzed by Western blot in CAL 27 cells at 4 h after treatment with 1 mmol/L phenformin (Phen), 200 nmol/L ISRIB, or DMSO as a control.

    ISRIB purchased from MedChemExpress. Usage Cited in: Int J Oral Sci. 2024 May 8;16(1):35.  [Abstract]

    CAL 27 cells were treated with 1 mmol/L phenformin (Phen) or with 200 nmol/L ISRIB or 1 μmol/L GSK2606414 or DMSO as a control. At 24 h, cells were collected and analyzed by the CCK-8 assay for cell viability.

    ISRIB purchased from MedChemExpress. Usage Cited in: J Virol. 2018 Jul 31;92(16). pii: e00600-18.  [Abstract]

    MARC-145 cells grown in six-well plates are treated with 0.5 mM Ars at 37°C for 1 h. The medium is changed to medium containing different concentrations of ISRIB and after 25 min, the cells are treated with puromycin, incubated at 37°C for 25 min, and lysed for western blotting analysis.

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    Beschreibung

    ISRIB is a brain-penetrant inhibitor of integrated stress response (ISR). Persistent activation of the ISR has been linked to the development of several neurological disorders as ISR represses translation through inhibiting eIF2B. ISRIB inhibits the ISR by promoting the nucleotide exchange activity of eIF2B and recovering the translation, and thus can be used for neurological disorders research[1][2][3].

    In Vitro

    ISRIB (200 nM, 2 h) blocks stress granule formation induced by eIF2α phosphorylation in U2OS cells[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    ISRIB Related Antibodies
    In Vivo

    ISRIB (0.25 mg/kg, i.p., daily) restores translation in prion-diseased mice at downstream of eIF2α phosphorylation, confering neuroprotection in prion-diseased mice [2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
    Molekulargewicht

    451.34

    Formel

    C22H24Cl2N2O4
    CAS. Nr.
    Appearance

    Solid

    Color

    Off-white to light yellow

    SMILES

    O=C(NC1CCC(NC(COC2=CC=C(Cl)C=C2)=O)CC1)COC3=CC=C(Cl)C=C3
    Versand

    Room temperature in continental US; may vary elsewhere.
    Speicherung
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Lösungsmittel & Löslichkeit
    In Vitro: 

    DMSO : 5.83 mg/mL (12.92 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.2156 mL 11.0781 mL 22.1562 mL
    5 mM 0.4431 mL 2.2156 mL 4.4312 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molaritätsrechner

    • Verdünnungsrechner

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo:

    The following protocol is derived from the literature and is for reference only. It is recommended to first try a small sample.

    • Protocol 1

      45% saline, 50% PEG 400, 5% DMSO

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Reinheit & Dokumentation

    Purity: 99.0%

    SDS (396 KB)

    COA (243 KB) HNMR (270 KB) RP-HPLC (251 KB) MS (228 KB)

    Handling Instructions (2659 KB)
    Verweise

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.2156 mL 11.0781 mL 22.1562 mL 55.3906 mL
    5 mM 0.4431 mL 2.2156 mL 4.4312 mL 11.0781 mL
    10 mM 0.2216 mL 1.1078 mL 2.2156 mL 5.5391 mL
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    ISRIB Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    ISRIB548470-11-7Biochemical Assay ReagentsEukaryotic Initiation Factor (eIF)neurological disordersISRinhibitoreIF2BInhibitorinhibit

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