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  3. Fluorescent Dye
  4. Laurdan

Laurdan is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound. Quantitation of generalized polarization (GP) of Laurdan can be used to identify phospholipid phase (Ex/Em = 370/440-490 nm).

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No. CAS : 74515-25-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 En stock
Solution
10 mM * 1 mL in DMSO En stock
Solid
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Revisión del cliente

Based on 23 publication(s) in Google Scholar

Other Forms of Laurdan:

Laurdan Related Antibodies

Top Publications Citing Use of Products

    Laurdan purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 29:e05436.  [Abstract]

    Quantification of membrane fluidity ( Laurdan 2 µM; 24 h) in SiHa cells exposed for 24 h to 15 µm ACOX1i (or DMSO as vehicle); Generalized polarization (GP) was calculated for ER membrane thanks to 1 µM ER‐tracker red staining.

    Laurdan purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Jul 22;44(7):115987.  [Abstract]

    Laurdan (10 μM). Membrane fluidity of parental, evolved, gene-deletion, and complementation strains treated with 8 μg/mL nitrofurantoin for 1 h.

    Laurdan purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2024 Aug 13:e2402693.  [Abstract]

    Representative flow cytometry plots of Annexin V and PI in wild-type (WT) and TMEM16F KO RMA cells after Listeria (Lm) infection, with histograms of Laurdan (30 min) dye fluorescence intensity showing designated gating regions.

    Laurdan purchased from MedChemExpress. Usage Cited in: J Oral Microbiol. 2023 Dec 10.

    Laurdan (10 μM; 30min) staining followed by the calculation of GP to represent cell membrane fluidity.

    Laurdan purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2022 Oct 16;79(11):552.  [Abstract]

    Bacterial cell membrane disruption by Laurdan probe staining. The fuorescence intensity of Laurdan (10 μM; 10 min) is recorded using a microplate reader with excitation at 350 nm and emission at 435 nm and 490 nm.

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    Descripciòn

    Laurdan is a membrane-permeable fluorescent probe that displays spectral sensitivity to the phospholipid phase of the cell membrane to which it is bound. Quantitation of generalized polarization (GP) of Laurdan can be used to identify phospholipid phase (Ex/Em = 370/440-490 nm).

    In Vitro

    Laurdan (from 1:1000 to 5:1000) acts as a biosensor to monitor changes in the membrane water content during cell migration in NIH3T3 cells[1].
    Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
    1. Laurdan staining solution preparation
    1.1 Preparation of stock solution
    Dissolve Laurdan in dimethyl sulfoxide (DMSO) to prepare a 1.8 mM stock solution.
    1.2 Preparation of working solution
    Dilute the Laurdan stock solution at a dilution of 1:1000.
    2. Laurdan staining
    2.1 NIH3T3 cells are cultured in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 5 mL Pen-Strep, and 2.5 mL 1 M HEPES.
    2.2 Freshly isolated NIH3T3 cells are seeded into 35 mm Mattek glass-bottom dishes coated with fibronectin.
    2.3 Cells are incubated with Laurdan for 40 min at 37°C.
    2.4 Observed using an LSM710 META laser scanning microscope.
    2.5 SimFCS software processing to calibrate the system and phasor diagram of FLIM data by measuring fluorescein (pH 11).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Laurdan Related Antibodies
    Peso molecular

    353.54

    Fòrmula

    C24H35NO
    No. CAS
    Appearance

    Solid

    Color

    White to yellow

    Emission (Em)

    440/490
    Excitation (Ex)

    370
    SMILES

    CCCCCCCCCCCC(C1=CC=C2C=C(N(C)C)C=CC2=C1)=O
    Envío

    Room temperature in continental US; may vary elsewhere.
    Almacenamiento

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvente y solubilidad
    In Vitro: 

    DMSO : 5 mg/mL (14.14 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.8285 mL 14.1427 mL 28.2853 mL
    5 mM 0.5657 mL 2.8285 mL 5.6571 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Calculadora de molaridad

    • Calculadora de dilución

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Pureza y Documentación

    Purity: 99.79%

    SDS (396 KB)

    COA (240 KB) HNMR (136 KB) RP-HPLC (137 KB) NP-HPLC (100 KB) LCMS (100 KB)

    Instrucciones de manejo (2659 KB)
    Referencias

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.8285 mL 14.1427 mL 28.2853 mL 70.7134 mL
    5 mM 0.5657 mL 2.8285 mL 5.6571 mL 14.1427 mL
    10 mM 0.2829 mL 1.4143 mL 2.8285 mL 7.0713 mL
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    Laurdan Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Laurdan74515-25-6Fluorescent DyeFluorescent probeInhibitorinhibitorinhibit

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    Inquiry Information

    Nombre del producto:
    Laurdan
    Cat. No.:
    HY-D0080
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