2. Academic Validation
  3. Blocking effects of heteroclitin D and gomisin J on L-type calcium channels in ventricular cells of guinea pig

Blocking effects of heteroclitin D and gomisin J on L-type calcium channels in ventricular cells of guinea pig

  • Acta Pharmacol Sin. 2000 Apr;21(4):373-6.
X M Zhang 1,  D F Chen,  X J He,  S Yang,  P Zheng,  M H Jiang
Affiliations

Affiliation

  • 1 Department of Pharmacology, Shanghai Medical University, Shanghai 200032, China. [email protected]
PMID: 11324471
Abstract

Aim: To study the effects of heteroclitin D (HD) and gomisin J (GJ), two Lignans from Kadsura medicinal Plants, on L-type calcium channels in ventricular cells of guinea pig.

Methods: The calcium currents were measured by whole-cell patch-clamp recording technique.

Results: HD 1 and 10 mumol/L decreased the L-type calcium current from (770 +/- 155) to (482 +/- 104) and (384 +/- 85) pA, respectively. GJ 10 mumol/L inhibited calcium current from (822 +/- 169) to (436 +/- 143) pA. Neither HD nor GJ affected the steady-state activation curve. But they had impact on steady-state inactivation curve. HD 10 mumol/L changed the half inactivation voltage (V0.5) from -22.7 to -40.9 mV, and slope factor (kappa) from 10.2 to 20.6 (n = 4 cells from 3 guinea pigs, P < 0.05). GJ 10 mumol/L changed the V0.5 from -17.7 to -33.3 mV, and kappa from 15.9 to 27.8 (n = 5 cells from 3 guinea pigs, P < 0.05).

Conclusion: HD and GJ inhibited L-type calcium channels.

Figures
Products