1. Academic Validation
  2. Transport of N-acetyl-D-mannosamine and N-acetyl-D-glucosamine in Escherichia coli K1: effect on capsular polysialic acid production

Transport of N-acetyl-D-mannosamine and N-acetyl-D-glucosamine in Escherichia coli K1: effect on capsular polysialic acid production

  • FEBS Lett. 2002 Jan 30;511(1-3):97-101. doi: 10.1016/s0014-5793(01)03318-x.
Beatriz Revilla-Nuin 1 Angel Reglero Honorina Martínez-Blanco Ignacio G Bravo Miguel A Ferrero Leandro B Rodríguez-Aparicio
Affiliations

Affiliation

  • 1 Departamento de Bioquímica y Biología Molecular, Universidad de León, Campus de Vegazana, 24007 León, Spain.
Abstract

N-Acetyl-D-mannosamine (ManNAc) and N-acetyl-D-glucosamine (GlcNAc) are the essential precursors of N-acetylneuraminic acid (NeuAc), the specific monomer of polysialic acid (PA), a Bacterial pathogenic determinant. Escherichia coli K1 uses both amino sugars as carbon sources and uptake takes place through the mannose phosphotransferase system transporter, a phosphoenolpyruvate-dependent phosphotransferase system that shows a broad range of specificity. Glucose, mannose, fructose, and glucosamine strongly inhibited the transport of these amino-acetylated sugars and GlcNAc and ManNAc strongly affected ManNAc and GlcNAc uptake, respectively. The ManNAc and the GlcNAc phosphorylation that occurs during uptake affected NeuAc synthesis in vitro. These findings account for the low in vivo PA production observed when E. coli K1 uses ManNAc or GlcNAc as a carbon source for growth.

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