2. Academic Validation
  3. Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

Satratoxin H generates reactive oxygen species and lipid peroxides in PC12 cells

  • Biol Pharm Bull. 2008 Jun;31(6):1115-20. doi: 10.1248/bpb.31.1115.
Punnee Nusuetrong 1,  Thitima Pengsuparp,  Duangdeun Meksuriyen,  Masaaki Tanitsu,  Haruhisa Kikuchi,  Michinao Mizugaki,  Ken-ichi Shimazu,  Yoshiteru Oshima,  Norimichi Nakahata,  Makoto Yoshida
Affiliations

Affiliation

  • 1 Department of Physiology, Faculty of Medicine, Srinakharinwirot University, Sukhumvit, Bangkok, Thailand.
PMID: 18520041 DOI: 10.1248/bpb.31.1115
Abstract

Satratoxin H, a mycotoxin, is thought to induce Apoptosis of PC12 cells through the activation of p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) in a glutathione (GSH)-sensitive manner. The present study was undertaken to further elucidate the mechanism by which satratoxin H induces cell death in PC12 cells. Satratoxin H caused Apoptosis of PC12 cells within 24-h, as determined by DNA fragmentation and flow cytometric analysis. Satratoxin H increased Reactive Oxygen Species (ROS) production and lipid peroxidation, as determined by malondialdehyde formation. These effects were attenuated by incubation of cells with GSH, suggesting that satratoxin H-induced increase in Apoptosis of serum-deprived PC12 cells may be partially mediated through the generation of ROS.

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