2. Academic Validation
  3. Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier

Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier

  • Protein Pept Lett. 2010 Feb;17(2):164-7. doi: 10.2174/092986610790225978.
Shinya Kida 1,  Yusuke Eto,  Yasuo Yoshioka,  Shinsaku Nakagawa,  Koichi Kawasaki,  Mitsuko Maeda
Affiliations

Affiliation

  • 1 Life Science Center, Faculty of Pharmaceutical Sciences, Kobe Gakuin University, Minatojima, Chuo-ku, Kobe 650-8586, Japan.
PMID: 20214640 DOI: 10.2174/092986610790225978
Abstract

Two cell-penetrating Peptides, a Pro-rich peptide derivative, acetyl-(Val-Arg-Leu-Pro-Pro-Pro)(3)-Gly-Cys amide, and an octaarginine derivative, acetyl-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Arg-Gly-Cys amide, were prepared by the solid phase method. Each peptide was coupled to the heterobifunctional cross-linking reagent, 6-maleimidohexanoic acid N-hydroxysuccinimide ester, and then conjugated to the Adenovirus vector containing luciferase gene. Peptide-modified Ad, as compared with wild-type Ad, exhibited excellent luciferase activity in B16BL6 cells.

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