6-Maleimidohexanoic acid N-hydroxysuccinimide ester (Synonyms: EMCS)

Name: 6-Maleimidohexanoic acid N-hydroxysuccinimide ester
Brand: MedChemExpress
SKU: HY-78961
Rating: 4.5 (1 reviews)

Cat. No.: HY-78961 Purity: 99.76%: FAQs
Data Sheet SDS COA Handling Instructions

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6-Maleimidohexanoic acid N-hydroxysuccinimide ester (EMCS) is a heterobifunctional cross-linking reagent. EMCS is used as a unique and useful reagent for preparation of hapten conjugate and enzyme immunoconjugates.

For research use only. We do not sell to patients.

6-Maleimidohexanoic acid N-hydroxysuccinimide ester Chemical Structure

CAS No. : 55750-63-5

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50 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
100 mg	USD 130	In-stock	Estimated Time of Arrival: December 31
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Based on 1 publication(s) in Google Scholar

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Description

IC₅₀ & Target

Non-cleavable Linker

In Vitro

6-Maleimidohexanoic acid N-hydroxysuccinimide ester is coupled to the peptides, and then conjugated to the Adenovirus vector containing luciferase gene as adenovirus vector carrier^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

308.29

Formula

C₁₄H₁₆N₂O₆

CAS No.

55750-63-5

Appearance

Solid

Color

White to off-white

SMILES

O=C(C=C1)N(CCCCCC(ON2C(CCC2=O)=O)=O)C1=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 230 mg/mL (746.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	3.2437 mL	16.2185 mL	32.4370 mL
5 mM	0.6487 mL	3.2437 mL	6.4874 mL
10 mM	0.3244 mL	1.6218 mL	3.2437 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 7.5 mg/mL (24.33 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 7.5 mg/mL (24.33 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 7.5 mg/mL (24.33 mM); Clear solution

This protocol yields a clear solution of ≥ 7.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (75.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.76%

Select Batch:

Data Sheet (273 KB) SDS (418 KB)

COA (249 KB) HNMR (113 KB) RP-HPLC (204 KB)

Handling Instructions (2659 KB)

References

[1]. Shinya Kida, et al. Studies on heterobifunctional cross-linking reagents, 6-maleimidohexanoic acid active esters. Chem Pharm Bull (Tokyo). 2007 Apr;55(4):685-7. [Content Brief]

[2]. Shinya Kida, et al. Evaluation of synthetic cell-penetrating peptides, Pro-rich peptide and octaargine derivatives, as adenovirus vector carrier. Protein Pept Lett. 2010 Feb;17(2):164-7. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	3.2437 mL	16.2185 mL	32.4370 mL	81.0925 mL
	5 mM	0.6487 mL	3.2437 mL	6.4874 mL	16.2185 mL
	10 mM	0.3244 mL	1.6218 mL	3.2437 mL	8.1092 mL
	15 mM	0.2162 mL	1.0812 mL	2.1625 mL	5.4062 mL
	20 mM	0.1622 mL	0.8109 mL	1.6218 mL	4.0546 mL
	25 mM	0.1297 mL	0.6487 mL	1.2975 mL	3.2437 mL
	30 mM	0.1081 mL	0.5406 mL	1.0812 mL	2.7031 mL
	40 mM	0.0811 mL	0.4055 mL	0.8109 mL	2.0273 mL
	50 mM	0.0649 mL	0.3244 mL	0.6487 mL	1.6218 mL
	60 mM	0.0541 mL	0.2703 mL	0.5406 mL	1.3515 mL
	80 mM	0.0405 mL	0.2027 mL	0.4055 mL	1.0137 mL
	100 mM	0.0324 mL	0.1622 mL	0.3244 mL	0.8109 mL

6-Maleimidohexanoic acid N-hydroxysuccinimide ester Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

6-Maleimidohexanoic acid N-hydroxysuccinimide ester55750-63-5EMCSADC LinkerAntibody-drug conjugates linkeraminosulfhydrylgroupscross-linkingreagentenzymeimmunoconjugateshaptenconjugateInhibitorinhibitorinhibit

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6-Maleimidohexanoic acid N-hydroxysuccinimide ester (Synonyms: EMCS)

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6-Maleimidohexanoic acid N-hydroxysuccinimide ester Related Antibodies

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Complete Stock Solution Preparation Table

6-Maleimidohexanoic acid N-hydroxysuccinimide ester Related Classifications

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