SPDP  (Synonyms: SPDP Crosslinker)

SPDP (SPDP Crosslinker) is a short-chain crosslinker for amine-to-sulfhydryl conjugation via NHS-ester and pyridyldithiol reactive groups that form cleavable (reducible) disulfide bonds with cysteine sulfhydryls. It is a glutathione cleavable ADC linker used for the antibody-drug conjugates (ADCs)^[1][2].

Equal amounts of anti-CD11c and anti-CTLA-4 Abs (in borate buffered saline; pH 8.5) are activated using SPDP.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

312.36

Solid

C₁₂H₁₂N₂O₄S₂

68181-17-9

O=C(ON1C(CCC1=O)=O)CCSSC2=NC=CC=C2

Room temperature in continental US; may vary elsewhere.

-20°C, protect from light, stored under nitrogen

*The compound is unstable in solutions, freshly prepared is recommended.

• [1]. Lobedanz S et al. A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug effluxpumps. Proc Natl Acad Sci U S A, 2007 Mar 13, 104(11):4612-7.  [Content Brief]

  [2]. Karumuthil-Melethil S et al. Dendritic cell-directed CTLA-4 engagement during pancreatic beta cell antigen presentation delays type 1diabetes. J Immunol 2010 Jun 15, 184(12):6695-708.  [Content Brief]