2. Academic Validation
  3. Synthesized tetrahydroisoquinoline alkaloid exerts anticancer effects at least in part by suppressing NF-κB-regulated proteins in A549 human lung cancer cells

Synthesized tetrahydroisoquinoline alkaloid exerts anticancer effects at least in part by suppressing NF-κB-regulated proteins in A549 human lung cancer cells

  • Oncol Rep. 2015 Mar;33(3):1141-6. doi: 10.3892/or.2014.3658.
Won Sup Lee 1,  Jeong Won Yun 1,  Arulkumar Nagappan 1,  Jing Nan Lu 1,  Min Jeong Kim 1,  Jeong-Hee Lee 2,  Dong Hoon Kim 3,  Yung Hyun Choi 4,  Hye Jung Kim 5,  Ki Churl Chang 4,  Jin-Myung Jung 6
Affiliations

Affiliations

  • 1 Department of Internal Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea.
  • 2 Department of Pathology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea.
  • 3 Department of Emergency Medicine, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea.
  • 4 Department of Pharmacology, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea.
  • 5 Department of Biochemistry, Dongeui University College of Oriental Medicine and Department of Biomaterial Control (BK21 program), Dongeui University Graduate School, Busan 614-052, Republic of Korea.
  • 6 Department of Neurosurgery, Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju 660-702, Republic of Korea.
PMID: 25482101 DOI: 10.3892/or.2014.3658
Abstract

CKD-712, a newly synthesized tetrahydroisoquinoline (THI) and an enantiomer (S form) of YS 49 (a derivative of higenamine) has been reported to suppress nuclear factor-κB (NF-κB) activity in normal cells. In the present study, we investigated the Anticancer effects of THI at a low concentration where CKD-712 did not induce cell death in normal cells. At the range of concentrations used, CKD-712 induced cell growth arrest, and inhibited the invasion and motility of A549 cells as determined by cell cycle analysis, a Matrigel-coated chamber assay, and a wound-healing assay, respectively. CKD-712 suppressed MMP-9, but not MMP-2 and Other NF-κB-regulated proteins involved in Cancer metastasis such as VEGF. Moreover, CKD-712 induced cell cycle arrest at G2M phase by suppressing cyclin A, cyclin B and CDK-1 expression. Taken together, these data suggested that CKD-712 may exert Anticancer effects by suppressing NF-κB pathways and inducing cell cycle arrest at G2M phase.

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