2. Academic Validation
  3. Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol

Diacylglycerol kinase η1 is a high affinity isozyme for diacylglycerol

  • FEBS Lett. 2015 May 8;589(11):1272-7. doi: 10.1016/j.febslet.2015.03.032.
Suguru Komenoi 1,  Fumika Takemura 1,  Hiromichi Sakai 1,  Fumio Sakane 2
Affiliations

Affiliations

  • 1 Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan.
  • 2 Department of Chemistry, Graduate School of Science, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan. Electronic address: [email protected].
PMID: 25862496 DOI: 10.1016/j.febslet.2015.03.032
Abstract

Diacylglycerol kinase (DGK) η plays important roles in various patho-physiological events such as oncogenesis. In this study, we performed an enzymological characterization of DGKη splice variant 1 (DGKη1). The Km value for diacylglycerol was 0.14 mol%. Intriguingly, the Km value of DGKη1 for diacylglycerol was at least 9-fold lower than those of Other DGK isozymes including DGKα, indicating that DGKη1 is a high affinity isozyme for diacylglycerol. Therefore, DGKη1 is a unique DGK isozyme, which may function at particular membrane sites where only low concentrations of diacylglycerol are supplied.

Keywords

ATP; Diacylglycerol; Diacylglycerol kinase; High affinity; Isozyme.

Figures
Products