2. Academic Validation
  3. Isolation of Lignan and Fatty Acid Derivatives from the Grains of Echinochloa utilis and Their Inhibition of Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Cells

Isolation of Lignan and Fatty Acid Derivatives from the Grains of Echinochloa utilis and Their Inhibition of Lipopolysaccharide-Induced Nitric Oxide Production in RAW 264.7 Cells

  • J Agric Food Chem. 2016 Jan 20;64(2):425-32. doi: 10.1021/acs.jafc.5b05638.
Duc Hung Nguyen 1 2,  Bing Tian Zhao 1,  Duc Dat Le 1,  Young Ho Yoon 3,  Jee Youn Ko 3,  Koan Sik Woo 3,  Do Youn Jun 4,  Young Ho Kim 4,  Mi Hee Woo 1
Affiliations

Affiliations

  • 1 College of Pharmacy, Catholic University of Daegu , Gyeongsan 38430, Republic of Korea.
  • 2 Phutho College of Pharmacy , Viettri City, Phutho Province 290000, Vietnam.
  • 3 Functional Cereal Crop Research Division, Department of Functional Crop, NICS, RDA , Milyang 50426, Republic of Korea.
  • 4 Laboratory of Immunobiology, School of Life Science and Biotechnology, College of Natural Sciences, Kyungpook National University , Daegu 39061, Republic of Korea.
PMID: 26725284 DOI: 10.1021/acs.jafc.5b05638
Abstract

Two new fatty acid derivatives, echinochlorins A (8) and B (9) and a racemic lignan, (±)-anti-1-(4-hydroxy-3-methoxyphenyl)-2-{4-[(E)-3-acetoxypropen-1-yl]-2-methoxyphenoxy}propan-1,3-diol 3-acetate (1), were isolated from Echinochloa utilis grains, along with six known Lignans (2-7) and two fatty acid derivatives (10, 11). Their structures were established by spectroscopic data analyses (IR, UV, HR-FABMS, GC-MS, and 1D and 2D NMR). The configuration of 1 was determined by Mosher's method. Compound 5 displayed potential inhibitory activity on lipopolysaccharide-induced NO production in macrophage RAW 264.7 cells with an IC50 value of 4.8 ± 0.5 μM. These isolated compounds in crude EtOH extract were also quantitated by HPLC.

Keywords

Echinochloa utilis; NO inhibitory activity; echinochlorins A and B; lignans.

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