2. Academic Validation
  3. Evaluating anti-oxidant potential of ganoderic acid A in STAT 3 pathway in prostate cancer

Evaluating anti-oxidant potential of ganoderic acid A in STAT 3 pathway in prostate cancer

  • Mol Biol Rep. 2016 Dec;43(12):1411-1422. doi: 10.1007/s11033-016-4074-z.
Balraj Singh Gill 1,  Sanjeev Kumar 1,  Navgeet 2
Affiliations

Affiliations

  • 1 Centre for Biosciences, Central University of Punjab, Bathinda, India.
  • 2 Department of Biotechnology, Doaba college, Jalandhar, Punjab, India. [email protected].
PMID: 27640015 DOI: 10.1007/s11033-016-4074-z
Abstract

Evaluating anti-oxidant potential of Ganoderic acid A in STAT 3 pathway in Prostate Cancer. Molecular docking and ADMET activities of different isoforms of ganoderic acid on STAT 3 pathway were performed by Maestro 9.6 (Schrödinger Inc). The ganoderic acid A is best-docked among isoforms which analyses the expression level of antioxidant and STAT 3 pathway in PC-3 cells. The receptor-based molecular docking reveals the best binding interaction of SH2 domain of STAT3 and ganoderic acid A with GScore (-6.134), kcal/mol, Lipophilic EvdW (-1.83), Electro (-1.1), Glide emodel (-31.857), H bond (1.98), MM-GBSA (-69.555). The molecular docking QikProp analyzed the absorption, distribution, metabolism, excretion, and toxicity (ADME/T). The ganoderic acid A is best-docked among isoforms which downregulates the expression of STAT 3 in PC-3 cells. Moreover, ganoderic acid A inhibits proliferation, viability, ROS, DPPH, and analyzed the expression of SOD1, SOD2, and SOD3 by Real time PCR in a PC-3 cell in a dose-dependent manner. Molecular docking revealed the mechanistic binding of Ganoderic acid A in STAT3 signaling, which inhibits the proliferation, viability, and ROS in PC-3 cells.

Keywords

Antioxidant; Cancer; Ganoderic acid; Molecular docking; STAT3.

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