2. Academic Validation
  3. Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper

  • Virus Res. 2017 Jan 2;227:49-56. doi: 10.1016/j.virusres.2016.09.013.
Seung Mok Ryu 1,  Jaeyoung Kwon 2,  Young Hye Seo 1,  Eun Gyeong Song 3,  Seong Su Hong 4,  Beom Seok Kim 1,  Jin Sung Hong 5,  Ki Hyun Ryu 6,  Dongho Lee 7
Affiliations

Affiliations

  • 1 Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea.
  • 2 Natural Constituents Research Center, Korea Institute of Science and Technology (KIST), Gangneung, 25451, Republic of Korea.
  • 3 Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea.
  • 4 Bio-Center, Gyeonggi Institute of Science & Technology Promotion, Suwon, 16229, Republic of Korea.
  • 5 Department of Applied Biology, College of Agriculture and Life Sciences, Kangwon National University, Chunchon, 24341, Republic of Korea.
  • 6 Plant Virus GenBank, Department of Horticulture, Biotechnology and Landscape Architecture, Seoul Women's University, Seoul, 01797, Republic of Korea. Electronic address: [email protected].
  • 7 Department of Biosystems and Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea. Electronic address: [email protected].
PMID: 27686478 DOI: 10.1016/j.virusres.2016.09.013
Abstract

A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify Antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host Plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10μM. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40μM.

Keywords

Antiviral agent; Brucea javanica; Capsicum annuum; Green fluorescent protein; Pepper mottle virus; Quassinoid.

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