2. Academic Validation
  3. Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

Locked nucleic acid (LNA) enhances binding affinity of triazole-linked DNA towards RNA

  • Chem Commun (Camb). 2017 Aug 3;53(63):8910-8913. doi: 10.1039/c7cc05159j.
Pawan Kumar 1,  Afaf H El-Sagheer 2,  Lynda Truong 1,  Tom Brown 1
Affiliations

Affiliations

  • 1 Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. [email protected].
  • 2 Department of Chemistry, University of Oxford, 12 Mansfield Road, Oxford, OX1 3TA, UK. [email protected] and Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
PMID: 28748236 DOI: 10.1039/c7cc05159j
Abstract

Oligonucleotides containing internal triazole-3'-LNA linkages bind to complementary RNA with similar affinity and specificity to unmodified Oligonucleotides, and significantly better than Oligonucleotides containing triazole alone. In contrast LNA on the 5'-side of the triazole does not stabilise duplexes. Triazole-LNA confers great resistance towards enzymatic degradation relative to LNA alone.

Figures
Products