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  3. Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells

Protein profiling identified key chemokines that regulate the maintenance of human pluripotent stem cells

  • Sci Rep. 2017 Nov 6;7(1):14510. doi: 10.1038/s41598-017-15081-6.
Zongmin Jiang 1 2,  Yonggang Li 3,  Xinglai Ji 2 4,  Yiyuli Tang 2 4,  Haijing Yu 1 2,  Lei Ding 1 2,  Min Yu 1 2,  Qinghua Cui 1 2,  Ming Zhang 5,  Yanping Ma 6,  Meizhang Li 7 8
Affiliations

Affiliations

  • 1 Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, China.
  • 2 Key Laboratory of Molecular Cancer Biology, Yunnan Education Department, Kunming, Yunnan, 650091, China.
  • 3 Department of Reproduction and Genetics, the First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China.
  • 4 State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming, Yunnan, 650091, China.
  • 5 Yunnan Key Laboratory of Stem Cell and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, 650500, China.
  • 6 Department of Reproduction and Genetics, the First People's Hospital of Yunnan Province, Kunming, Yunnan, 650032, China. [email protected].
  • 7 Laboratory of Biochemistry and Molecular Biology, School of Life Sciences, Yunnan University, Kunming, Yunnan, 650091, China. [email protected].
  • 8 Key Laboratory of Molecular Cancer Biology, Yunnan Education Department, Kunming, Yunnan, 650091, China. [email protected].
PMID: 29109449 DOI: 10.1038/s41598-017-15081-6
Abstract

Microenvironment (or niche)-providing chemokines regulate many important biological functions of tissue-specific stem cells. However, to what extent chemokines influence human pluripotent stem cells (hPSCs) is not yet completely understood. In this study, we applied protein array to screen chemokines found within the cytokine pool in the culture supernatant of hPSCs. Our results showed that chemokines were the predominant supernatant components, and came from three sources: hPSCs, feeder cells, and culture media. Chemotaxis analysis of IL-8, SDF-1α, and IP-10 suggested that chemokines function as uniform chemoattractants to mediate in vitro migration of the hPSCs. Chemokines mediate both differentiated and undifferentiated states of hPSCs. However, balanced chemokine signaling tends to enhance their stemness in vitro. These results indicate that chemokines secreted from both stem cells and feeder cells are essential to mobilize hPSCs and maintain their stemness.

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