2. Academic Validation
  3. Cryoprotectant toxicity in Caenorhabditis elegans

Cryoprotectant toxicity in Caenorhabditis elegans

  • Cryobiology. 2019 Feb;86:71-76. doi: 10.1016/j.cryobiol.2018.12.002.
Patricia M Tedesco 1,  Garrett J Schumacher 1,  Thomas E Johnson 2
Affiliations

Affiliations

  • 1 Institute for Behavioral Genetics, University of Colorado, Boulder, USA.
  • 2 Institute for Behavioral Genetics, University of Colorado, Boulder, USA; Department of Integrative Physiology, University of Colorado, Boulder, USA. Electronic address: [email protected].
PMID: 30527584 DOI: 10.1016/j.cryobiol.2018.12.002
Abstract

We have looked at the effects of the cryoprotectant M22 upon viability in the model organism C. elegans. M22 is a well-known vitrification solution which has been successfully used in the laboratory to preserve organs destined for transplantation. M22 reduces survival of C. elegans in a concentration-dependent manner. M22 at concentrations of 10% (v/v) or higher inhibits progeny production and development. A few mutants in the ILS (insulin-like signaling) pathway of C. elegans are more resistant to the toxic effect of M22 compared to wild-type worms. Afatinib, an anti-cancer drug, protects against M22 toxicity. Afatinib by itself does not increase longevity.

Keywords

Afatinib; Caenorhabditis elegans; Cryopreservation; Cryoprotectant toxicity; ILS mutants; M22; Stress resistance.

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