2. Academic Validation
  3. Rohitukine inhibits NF-κB activation induced by LPS and other inflammatory agents

Rohitukine inhibits NF-κB activation induced by LPS and other inflammatory agents

  • Int Immunopharmacol. 2019 Apr:69:34-49. doi: 10.1016/j.intimp.2019.01.015.
Amarinder Singh 1,  Pankaj Chibber 2,  Praveen Kolimi 3,  Tanveer Ahmad Malik 4,  Nitika Kapoor 5,  Amit Kumar 6,  Narinder Kumar 7,  Sumit G Gandhi 8,  Surjeet Singh 2,  Sheikh Tasduq Abdullah 4,  Ram Vishwakarma 2,  Gurdarshan Singh 9
Affiliations

Affiliations

  • 1 Academy of Scientific & Innovative Research (AcSIR), New Delhi, India; PK-PD-Toxicology and Formulation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India. Electronic address: [email protected].
  • 2 Academy of Scientific & Innovative Research (AcSIR), New Delhi, India; PK-PD-Toxicology and Formulation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India.
  • 3 NIPER, Ahmedabad, India.
  • 4 Cancer Pharmacology, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India.
  • 5 Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India.
  • 6 PK-PD-Toxicology and Formulation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India.
  • 7 Govt. Science College, Canal Road, Jammu 180001, J & K, India.
  • 8 PK-PD-Toxicology and Formulation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India; Plant Biotechnology Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India.
  • 9 Academy of Scientific & Innovative Research (AcSIR), New Delhi, India; PK-PD-Toxicology and Formulation Division, CSIR-Indian Institute of Integrative Medicine, Canal Road, Jammu 180001, J & K, India. Electronic address: [email protected].
PMID: 30665042 DOI: 10.1016/j.intimp.2019.01.015
Abstract

Rohitukine (referred to as RHK) is a bioactive chromone alkaloid isolated from the leaves of plant Dysoxylum binectariferum, which has been reported to possess diverse pharmacological properties for the treatment of inflammatory bowel disease (IBD), diarrhoea and anti-lipidemic. However, the underlying mechanism by which RHK exerts its anti-inflammatory activity has not yet demonstrated. This study aimed to elucidate the anti-inflammatory mechanism of RHK using lipopolysaccharide (LPS) - stimulated J774A.1 macrophage cells and in-vivo inflammatory models. Results demonstrated that RHK treatment could significantly decrease the LPS-induced production of nitric oxide, prostaglandin E2 (PGE2), interleukins (ILs) and tumour necrosis factor (TNF)-α in J774A.1 cells. Molecular studies revealed that RHK inhibited the activation of upstream mediator nuclear factor-κB by suppressing the phosphorylation of IκBα and p65. In in-vivo experiments showed prominent anti-inflammatory activity of RHK. Thus, RHK could be considered as a promising candidate for the treatment of inflammatory diseases.

Keywords

Dysoxylum binectariferum; Inflammation; Macrophage; NF-κB; Rohitukine.

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