2. Academic Validation
  3. Iguratimod (T-614) attenuates severe acute pancreatitis by inhibiting the NLRP3 inflammasome and NF-κB pathway

Iguratimod (T-614) attenuates severe acute pancreatitis by inhibiting the NLRP3 inflammasome and NF-κB pathway

  • Biomed Pharmacother. 2019 Nov;119:109455. doi: 10.1016/j.biopha.2019.109455.
Chaoqun Hou 1,  Xiaole Zhu 1,  Chenyuan Shi 1,  Yunpeng Peng 1,  Dongya Huang 1,  Qiang Li 2,  Yi Miao 3
Affiliations

Affiliations

  • 1 Pancreas Centre, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, Province, China; Pancreas Institute, Nanjing Medical University, Nanjing, Jiangsu Province, China.
  • 2 Pancreas Centre, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, Province, China; Pancreas Institute, Nanjing Medical University, Nanjing, Jiangsu Province, China. Electronic address: [email protected].
  • 3 Pancreas Centre, First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, Province, China; Pancreas Institute, Nanjing Medical University, Nanjing, Jiangsu Province, China. Electronic address: [email protected].
PMID: 31541854 DOI: 10.1016/j.biopha.2019.109455
Abstract

Severe acute pancreatitis (SAP) is an acute abdominal disease that can develop locally to the multiple organs. It is characterized by pancreatic tissue self-digestion, and the rapid release of inflammatory cytokines, which play a dominant role in local or even systemic inflammation. In this study, we investigate the protective effect of T-614 against SAP induced by cerulein plus LPS in mice. Biochemical markers associated with pancreatitis in serum such as inflammatory cytokines, amylase and Lipase activities were measured. Related proteins of NLRP3 inflammasome and NF-κB signaling pathway were evaluated by western blotting. Hematoxylin-eosin staining (HE) and immunohistochemistry (IHC) were used to evaluate changes of inflammation in pancreatic tissue. T-614 significantly alleviated the elevation markers of pancreatitis and suppresses the pancreatic tissue damage, including histopathological and molecular manifestations. In conclusion, T-614 plays a protective role in experimental SAP mice model via anti-inflammatory effects.

Keywords

COX-2; Iguratimod; NF-κB pathway; NLRP3; Severe acute pancreatitis.

Figures
Products