1. Academic Validation
  2. Hyperoside Induces Breast Cancer Cells Apoptosis via ROS-Mediated NF-κB Signaling Pathway

Hyperoside Induces Breast Cancer Cells Apoptosis via ROS-Mediated NF-κB Signaling Pathway

  • Int J Mol Sci. 2019 Dec 24;21(1):131. doi: 10.3390/ijms21010131.
Jinxia Qiu 1 Tao Zhang 1 Xinying Zhu 1 Chao Yang 1 Yaxing Wang 2 Ning Zhou 1 Bingxin Ju 1 Tianhong Zhou 1 Ganzhen Deng 1 Changwei Qiu 1
Affiliations

Affiliations

  • 1 Department of Clinical Veterinary Medicine, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
  • 2 College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China.
Abstract

Hyperoside (quercetin 3-o-β-d-galactopyranoside) is one of the flavonoid glycosides with anti-inflammatory, antidepressant, and anti-cancer effects. But it remains unknown whether it had effects on breast Cancer. Here, different concentrations of hyperoside were used to explore its therapeutic potential in both breast Cancer cells and subcutaneous homotransplant mouse model. CCK-8 and wound healing assays showed that the viability and migration capability of Michigan Cancer Foundation-7 (MCF-7) and 4T1 cells were inhibited by hyperoside, while the Apoptosis of cells were increased. Real-time quantitative PCR (qRT-PCR) and western blot analysis were used to detect mRNA and the protein level, respectively, which showed decreased levels of B cell lymphoma-2 (Bcl-2) and X-linked inhibitor of Apoptosis (XIAP), and increased levels of Bax and cleaved Caspase-3. After exploration of the potential mechanism, we found that Reactive Oxygen Species (ROS) production was reduced by the administration of hyperoside, which subsequently inhibited the activation of NF-κB signaling pathway. Tumor volume was significantly decreased in subcutaneous homotransplant mouse model in hyperoside-treated group, which was consistent with our study in vitro. These results indicated that hyperoside acted as an Anticancer drug through ROS-related Apoptosis and its mechanism included activation of the Bax-caspase-3 axis and the inhibition of the NF-κB signaling pathway.

Keywords

ROS; apoptosis; breast cancer; hyperoside; nuclear transcription factor-κB (NF-κB).

Figures
Products