Induction of autophagy and suppression of type I IFN secretion by CSFV

Macroautophagy/Autophagy plays an essential role in cellular responses to pathogens. However, the precise mechanisms and signaling pathways that modulate cellular Autophagy in classical swine fever virus (CSFV)-infected host cells have not been confirmed. In this study, we showed that CSFV Infection inhibits the phosphorylation of mTOR (mechanistic target of rapamycin kinase), subsequently leading to Autophagy initiation. We also show that MAPK/ERK (mitogen-activated protein kinase) signaling is involved in CSFV-induced Autophagy. The CSFV-induced inhibition of Akt/PKB (Akt serine/threonine kinase)-MTOR was observed to be partially responsible for the mTOR inactivation and subsequent Autophagy initiation. Moreover, the CAMKK2/CaMKKβ (calcium/Calmodulin dependent protein kinase kinase 2)-PRKAA/AMPK (protein kinase AMP-activated catalytic subunit alpha) axis was found to be involved in CSFV-induced Autophagy. Meanwhile, CSFV non-structural protein NS5A induced Autophagy via the CAMKK2-PRKAA-MTOR signaling pathway but not the AKT-MTOR or MAPK1/ERK2-MAPK3/ERK1-MTOR pathway. Although the AKT-MTOR pathway also plays an important role in the induction of Autophagy by CSFV. We also found the interaction between HSP90AB1/HSPCB and NS5A by tandem affinity purification/liquid chromatography-mass spectrometry (LC-MS) and immunoprecipitation. Furthermore, the CSFV-induced [Ca²⁺]_cyto increase potently induced Autophagy through CAMKK2 and PRKAA. Moreover, we isolated and identified the BECN1/Beclin 1 protein complexes by tandem affinity purification/LC-MS and immunoprecipitation, the interaction between BECN1 and MAVS was confirmed by immunoprecipitation, laser scanning confocal microscope technology, and GST affinity-isolation experiments. Furthermore, CSFV-mediated Autophagy suppressing type I IFN production is related to the interaction between MAVS and BECN1. Finally, the modulation of Autophagy induction pathways by different Autophagy regulatory factors significantly affected the replication of CSFV.Abbreviations: AKT: Akt serine/threonine kinase; AMPK: Adenosine monophosphate-activated protein kinase; CAMKK2: Calcium/Calmodulin dependent protein kinase kinase 2; CSFV: Classical swine fever virus; HRP: Horseradish peroxidase; HSP90AB1: Heat shock protein 90 alpha family class B member 1; IFN: Interferon; ISGs: IFN-stimulated genes; LC-MS: Liquid chromatography-mass spectrometry; MAP1LC3/LC3: Microtubule associated protein 1 LIGHT chain 3; MAPK: Mitogen-activated protein kinase; MAVS: Mitochondrial Antiviral signaling protein; MOI: Multiplicity of infection; MTOR: Mechanistic target of rapamycin kinase; PBS: Phosphate-buffered saline; PRKAA: Protein kinase AMP-activated catalytic subunit alpha; shRNA: short hairpin RNA.