1. Membrane Transporter/Ion Channel
  2. Potassium Channel
  3. BAPTA-AM

BAPTA-AM 

Cat. No.: HY-100545 Purity: 99.60%
Handling Instructions

BAPTA-AM est un chélateur Ca2+ perméable à la membrane bien connu. BAPTA-AM inhibe les canaux hERG, hKv1.3 et hKv1.5 dans les cellules HEK 293 avec IC50 de 1,3 μM, 1,45 μM et 1,23 μM, respectivement.

BAPTA-AM ist ein bekannter membrandurchlässiger Ca2+ chelator. BAPTA-AM hemmt hERG-Kanäle, hKv1.3 und hKv1.5-Kanäle in HEK 293 Zellen mit IC50s von 1,3 μM, 1,45 μM und 1,23 μM.

BAPTA-AM is a well-known membrane permeable Ca2+ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50s of 1.3 μM, 1.45 μM and 1.23 μM, respectively.

For research use only. We do not sell to patients.

BAPTA-AM Chemical Structure

BAPTA-AM Chemical Structure

CAS No. : 126150-97-8

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5 mg USD 90 In-stock
Estimated Time of Arrival: December 31
10 mg USD 150 In-stock
Estimated Time of Arrival: December 31
50 mg USD 450 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 15 publication(s) in Google Scholar

Top Publications Citing Use of Products

    BAPTA-AM purchased from MCE. Usage Cited in: Oncotarget. 2016 Feb 9;7(6):6711-26.

    Under conditions of glucose limitation, BAPTA-AM treatment prevents HBx-induced phosphorylation of AMPK and ACC (A). This inhibitory effect is also observed in HBV-infected HepG2.2.15 cells in a concentration-dependent manner (B), implying that HBx-mediated mitochondrial calcium channels are required for activation of the AMPK pathway under such stressful conditions.

    BAPTA-AM purchased from MCE. Usage Cited in: Part Fibre Toxicol. 2018 Oct 19;15(1):39.

    Total proteins of BV2 cells are extracted, and the levels of P2X7, NF-κB, ERK and p38 signaling pathway molecules are analyzed via Western Blot.
    • Biological Activity

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    • References

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    Description

    BAPTA-AM is a well-known membrane permeable Ca2+ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50s of 1.3 μM, 1.45 μM and 1.23 μM, respectively[1].

    IC50 & Target

    Ca2+ chelator[1]
    IC50: 1.3 μM (hERG channel, in HEK 293 cells), 1.45 μM (hKv1.3, in HEK 293 cells), 1.23 μM (hKv1.5, in HEK 293 cells)[1]

    In Vitro

    BAPTA-AM inhibits neuronal Ca2+-activated K+ channel currents, and up-regulates the decreased cardiac sodium current (INa) density by chelating intracellular Ca2+[1].
    BAPTA-AM (BAPTA/AM), an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. BAPTA-AM prevents free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA-AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10 μM BAPTA-AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA-AM for 48-hr evoke dose-dependent neuronal damage[2].

    Molecular Weight

    764.68

    Formula

    C₃₄H₄₀N₂O₁₈

    CAS No.

    126150-97-8

    SMILES

    O=C(OCOC(C)=O)CN(C(C=CC=C1)=C1OCCOC(C=CC=C2)=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    DMSO : 150 mg/mL (196.16 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3077 mL 6.5387 mL 13.0774 mL
    5 mM 0.2615 mL 1.3077 mL 2.6155 mL
    10 mM 0.1308 mL 0.6539 mL 1.3077 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution

    *All of the co-solvents are provided by MCE.
    References
    Cell Assay
    [1]

    Neuronal injury is quantitatively estimated by measuring lactate dehydrogenase (LDH) released from damaged cells into the bathing medium 24- or 48-hr after the 10 μM BAPTA/AM treatment. The morphological findings are confirmed by staining with neuron-specific enolase (NSE) antibody and tryphan blue[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.60%

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    Keywords:

    BAPTA-AMPotassium ChannelKcsAInhibitorinhibitorinhibit

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    Product Name:
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