1. Biochemical Assay Reagents

BAPTA-AM 

Cat. No.: HY-100545 Purity: 99.60%
Handling Instructions

BAPTA-AM is an intracellular calcium chelator.

For research use only. We do not sell to patients.

BAPTA-AM Chemical Structure

BAPTA-AM Chemical Structure

CAS No. : 126150-97-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 71 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 336 In-stock
Estimated Time of Arrival: December 31
100 mg USD 576 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

    BAPTA-AM purchased from MCE. Usage Cited in: Oncotarget. 2016 Feb 9;7(6):6711-26.

    Under conditions of glucose limitation, BAPTA-AM treatment prevents HBx-induced phosphorylation of AMPK and ACC (A). This inhibitory effect is also observed in HBV-infected HepG2.2.15 cells in a concentration-dependent manner (B), implying that HBx-mediated mitochondrial calcium channels are required for activation of the AMPK pathway under such stressful conditions.

    BAPTA-AM purchased from MCE. Usage Cited in: Part Fibre Toxicol. 2018 Oct 19;15(1):39.

    Total proteins of BV2 cells are extracted, and the levels of P2X7, NF-κB, ERK and p38 signaling pathway molecules are analyzed via Western Blot.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References

    Description

    BAPTA-AM is an intracellular calcium chelator.

    IC50 & Target

    calcium chelator[1]

    In Vitro

    The permeable calcium chelator BAPTA/AM is known to prevent free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA/AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10μM BAPTA/AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA/AM for 48-hr evoke dose-dependent neuronal damage[1].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3077 mL 6.5387 mL 13.0774 mL
    5 mM 0.2615 mL 1.3077 mL 2.6155 mL
    10 mM 0.1308 mL 0.6539 mL 1.3077 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    Neuronal injury is quantitatively estimated by measuring lactate dehydrogenase (LDH) released from damaged cells into the bathing medium 24- or 48-hr after the 10 μM BAPTA/AM treatment. The morphological findings are confirmed by staining with neuron-specific enolase (NSE) antibody and tryphan blue[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    764.68

    Formula

    C₃₄H₄₀N₂O₁₈

    CAS No.

    126150-97-8

    SMILES

    O=C(OCOC(C)=O)CN(C(C=CC=C1)=C1OCCOC(C=CC=C2)=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

    Storage
    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: 99.60%

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    The molarity calculator equation

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    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

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    Product Name:
    BAPTA-AM
    Cat. No.:
    HY-100545
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    BAPTA-AM

    Cat. No.: HY-100545