BAPTA-AM
Based on 146 publication(s) in Google Scholar
BAPTA-AM is a well-known membrane permeable Ca2+ chelator. BAPTA-AM inhibits hERG channels, hKv1.3 and hKv1.5 channels in HEK 293 cells with IC50s of 1.3 μM, 1.45 μM and 1.23 μM, respectively.
For research use only. We do not sell to patients.
- Purity: 99.23%
- CAS No.: 126150-97-8
- Formula: C34H40N2O18
- Molecular Weight:764.68
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Publications Citing Use of MedChemExpress (MCE) BAPTA-AM
More- Signal Transduct Target Ther. 2022 Feb 16;7(1):46. [Abstract]
- Nature. 2025 Oct;646(8086):913-924. [Abstract]
- Cancer Cell. 2024 Dec 9;42(12):2082-2097.e10. [Abstract]
- Cell Metab. 2026 Jul 7;38(7):1367-1384.e9. [Abstract]
- Adv Mater. 2025 Oct 1:e09410. [Abstract]
- Adv Mater. 2024 Mar 24:e2401384. [Abstract]
- Nat Immunol. 2019 Apr;20(4):433-446. [Abstract]
- Cell Stem Cell. 2022 Nov 3;29(11):1531-1546.e7. [Abstract]
- J Extracell Vesicles. 2022 Oct;11(10):e12274. [Abstract]
- Bone Res. 2022 Mar 8;10(1):25. [Abstract]
- Autophagy. 2025 Feb;21(2):352-373. [Abstract]
- Autophagy. 2024 Nov;20(11):2388-2404. [Abstract]
- Autophagy. 2021 Apr;17(4):925-947. [Abstract]
- Nat Commun. 2023 Feb 6;14(1):642. [Abstract]
- Nat Commun. 2021 May 18;12(1):2915. [Abstract]
- J Adv Res. 2025 Jan 25:S2090-1232(25)00059-1. [Abstract]
- Redox Biol. 2026 Jun 11:95:104255. [Abstract]
- Redox Biol. 2026 Mar:90:104059. [Abstract]
- J Nanobiotechnology. 2025 Nov 25;23(1):737. [Abstract]
- Acta Pharm Sin B. 2025 Aug;15(8):4078-4095. [Abstract]
- J Exp Clin Cancer Res. 2024 Oct 3;43(1):277. [Abstract]
- Adv Sci (Weinh). 2025 Nov 10:e10991. [Abstract]
- Adv Sci (Weinh). 2025 Aug 7:e09208. [Abstract]
- Adv Sci (Weinh). 2025 Jul 25:e15313. [Abstract]
- Adv Sci (Weinh). 2023 Mar;10(7):e2203869. [Abstract]
- Cell Death Dis. 2021 Dec 14;12(12):1159. [Abstract]
- Cell Death Dis. 2021 Oct 13;12(10):942. [Abstract]
- Small. 2025 Mar;21(12):e2408139. [Abstract]
- Cancer Lett. 2023 Nov 28:577:216435. [Abstract]
- Cancer Lett. 2021 Nov 1:520:26-37. [Abstract]
- Int J Biol Sci. 2026 Jan 15;22(4):1834-1851. [Abstract]
- Int J Biol Sci. 2023 Jun 4;19(9):2914-2933. [Abstract]
- Cell Commun Signal. 2024 Nov 7;22(1):534. [Abstract]
- Phytomedicine. 2026 Jun 11:159:158409. [Abstract]
- Phytomedicine. 2026 May 26. [Abstract]
- Phytomedicine. 2025 Jul:142:156632. [Abstract]
- Mater Today Bio. 2025 Jan 13:31:101489. [Abstract]
- Part Fibre Toxicol. 2018 Oct 19;15(1):39. [Abstract]
- eGastroenterology. 2026 Mar 31;4(1):e100348. [Abstract]
- Cell Death Discov. 2025 Jun 21;11(1):284. [Abstract]
- J Rare Earths. 2026 May 9.
- Proc Natl Acad Sci U S A. 2025 Sep 2;122(35):e2513155122. [Abstract]
- Food Res Int. 2025 Dec;222(Pt 1):117656. [Abstract]
- Small Methods. 2025 Mar;9(3):e2401144. [Abstract]
- Int J Biol Macromol. 2024 Sep 23;280(Pt 3):135876. [Abstract]
- Sens Actuators B Chem. 2026 Jun 7;466:140329.
- Free Radic Biol Med. 2025 Dec 16:244:380-394. [Abstract]
- Free Radic Biol Med. 2025 Oct 23:S0891-5849(25)01299-7. [Abstract]
- EMBO Mol Med. 2025 Oct;17(10):2586-2611. [Abstract]
- EMBO Mol Med. 2023 Jan 11;15(1):e16373. [Abstract]
- Clin Transl Med. 2020 Oct;10(6):e205. [Abstract]
- Cell Rep. 2026 Jan 3;45(1):116683. [Abstract]
- Cell Rep. 2025 Dec 5;44(12):116659. [Abstract]
- Aging Cell. 2025 Apr;24(4):e14440. [Abstract]
- Cell Rep. 2024 Dec 21;44(1):115094. [Abstract]
- Chin Med. 2026 Apr 29;21(1):123. [Abstract]
- Virulence. 2026 Dec;17(1):2670939. [Abstract]
- Virulence. 2025 Dec;16(1):2490208. [Abstract]
- Virulence. 2025 Dec;16(1):2466482. [Abstract]
- Virulence. 2025 Oct 28:2580150. [Abstract]
- NanoImpact. 2025 Jul:39:100572. [Abstract]
- J Agric Food Chem. 2025 Apr 10. [Abstract]
- CNS Neurosci Ther. 2025 Dec;31(12):e70715. [Abstract]
- Ecotoxicol Environ Saf. 2024 Jan 1:269:115906. [Abstract]
- Ecotoxicol Environ Saf. 2023 Sep 15:263:115262. [Abstract]
- Ecotoxicol Environ Saf. 2023 Jun 1:257:114940. [Abstract]
- Biochem Pharmacol. 2026 Jun 12;251(Pt 2):118163. [Abstract]
- Biochem Pharmacol. 2025 Sep 19;242(Pt 3):117354. [Abstract]
- Biochem Pharmacol. 2024 Nov 10;230(Pt 3):116618. [Abstract]
- Cell Mol Life Sci. 2024 Jan 22;81(1):49. [Abstract]
- Life Sci. 2019 Jul 1:228:85-97. [Abstract]
- Food Biosci. 2025 Mar.
- Food Biosci. 2025 Jan.
- RSC Adv. 2019 Aug 13;9(43):25107-25118. [Abstract]
- Cells. 2024 Apr 23;13(9):728. [Abstract]
- Cancer Immunol Immunother. 2026 May 8;75(7):175. [Abstract]
- Commun Biol. 2025 Jul 29;8(1):1118. [Abstract]
- Commun Biol. 2025 Mar 12;8(1):417. [Abstract]
- Cell Biol Toxicol. 2024 Dec 27;41(1):18. [Abstract]
- Acta Physiol. 2023 Apr;237(4):e13926. [Abstract]
- Cell Oncol (Dordr). 2026 Jun 23. [Abstract]
- Cell Oncol (Dordr). 2026 Jun 6. [Abstract]
- Int Immunopharmacol. 2026 Feb 1:170:116072. [Abstract]
- Int J Mol Sci. 2026 Jan 30;27(3):1425. [Abstract]
- Int Immunopharmacol. 2025 Oct 30:164:115325. [Abstract]
- Int Immunopharmacol. 2024 Sep 16;142(Pt B):113170. [Abstract]
- Int J Mol Sci. 2024 Jul 1;25(13):7254. [Abstract]
- Int Immunopharmacol. 2024 Feb 15:128:111418. [Abstract]
- Biomolecules. 2022 Nov 29;12(12):1778. [Abstract]
- Mucosal Immunol. 2020 Nov;13(6):931-945. [Abstract]
- Front Pharmacol. 2025 Nov 18:16:1697815. [Abstract]
- mBio. 2024 Dec 11;15(12):e0213724. [Abstract]
- Int Dent J. 2026 Jun 27;76(5):109698. [Abstract]
- Toxicology. 2025 Nov:517:154212. [Abstract]
- Mol Med Rep. 2024 Jul;30(1):110. [Abstract]
- Sci Rep. 2026 May 18. [Abstract]
- Cell Calcium. 2025 Dec:132:103071. [Abstract]
- Pestic Biochem Physiol. 2026 Jun 17;222:107215.
- Mol Cell Biochem. 2025 Aug 3. [Abstract]
- Oncol Rep. 2024 Feb;51(2):29. [Abstract]
- Acta Biochim Biophys Sin (Shanghai). 2026 May 25. [Abstract]
- Biosci Rep. 2019 Jul 2;39(7):BSR20190516. [Abstract]
- Aquaculture. 15 October 2022, 738445.
- FASEB J. 2025 Sep 30;39(18):e71061. [Abstract]
- Front Physiol. 2022 Jan 5:12:736920. [Abstract]
- Membranes. 2023 Aug 5;13(8):720. [Abstract]
- J Virol. 2025 May 20;99(5):e0007825. [Abstract]
- BMC Cancer. 2025 May 28;25(1):956. [Abstract]
- J Virol. 2023 May 31;97(5):e0029223. [Abstract]
- BMC Cancer. 2022 Dec 19;22(1):1327. [Abstract]
- J Cell Physiol. 2024 Aug;239(8):e31290. [Abstract]
- J Cell Physiol. 2021 Aug;236(8):5818-5831. [Abstract]
- Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1;321(6):L1161-L1182. [Abstract]
- J Bioenerg Biomembr. 2026 Feb 11;58(1):1. [Abstract]
- J Pharm Pharmacol. 2023 Apr 17;75(5):645-654. [Abstract]
- Toxicol Appl Pharmacol. 2024 May 20:487:116976. [Abstract]
- Cell Biochem Funct. 2026 Apr;44(4):e70214. [Abstract]
- Exp Cell Res. 2019 Jun 15;379(2):191-202. [Abstract]
- Insect Mol Biol. 2025 May 26. [Abstract]
- J Immunol Res. 2026;2026(1):e5542735. [Abstract]
- J Appl Toxicol. 2024 Jun;44(6):818-832. [Abstract]
- Oral Dis. 2024 Apr;30(3):1416-1428. [Abstract]
- BMC Mol Cell Biol. 2026 Jan 18. [Abstract]
- J Pharmacol Sci. 2025 Jul;158(3):219-230. [Abstract]
- Hum Cell. 2024 Nov;37(6):1649-1662. [Abstract]
- Virology. 2020 Jan 2:539:38-48. [Abstract]
- Chin J Traumatol. 2026 Mar 7:S1008-1275(26)00041-6. [Abstract]
- Biochem Biophys Res Commun. 2025 Mar 5:751:151414. [Abstract]
- Endocr J. 2022 Apr 28;69(4):385-397. [Abstract]
- Biocell. 2026 Jan 23.
- bioRxiv. 2026 Jun 5:2026.06.04.730242. [Abstract]
- Northwestern University. 2026.
- SSRN. 2026 Apr 20.
- bioRxiv. 2026 Mar 26.
- Res Sq. 2026 Mar 11.
- bioRxiv. 2026 Mar 6.
- Res Sq. 2025 Oct 6.
- SSRN. 2025 Aug 13.
- bioRxiv. 2025 Mar 19:2025.03.19.644113. [Abstract]
- bioRxiv. 2024 September 07.
- bioRxiv. 2024 June 14.
- bioRxiv. 2024 Jan 23.
- University of Padua. 2022.
- Biomed Pharmacother. 2022 Sep:153:113292. [Abstract]
- Research Square Preprint. 2020 Jun.
- Oncotarget. 2016 Feb 9;7(6):6711-26. [Abstract]
Customer Validation & Images
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Biological Activity
Ca2+ chelator[1]
IC50: 1.3 μM (hERG channel, in HEK 293 cells), 1.45 μM (hKv1.3, in HEK 293 cells), 1.23 μM (hKv1.5, in HEK 293 cells)[1]
BAPTA-AM inhibits neuronal Ca2+-activated K+ channel currents, and up-regulates the decreased cardiac sodium current (INa) density by chelating intracellular Ca2+[1].
BAPTA-AM (BAPTA/AM), an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. BAPTA-AM prevents free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA-AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10 μM BAPTA-AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA-AM for 48-hr evoke dose-dependent neuronal damage[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 126150-97-8
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Appearance Solid
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Molecular Weight 764.68
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Formula C34H40N2O18
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Color White to off-white
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SMILES
O=C(OCOC(C)=O)CN(C(C=CC=C1)=C1OCCOC(C=CC=C2)=C2N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (146)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. [Abstract]2022 Feb 16;7(1):46. PMID: 35169126 -
Nature
2025 Oct;646(8086):913-924. PMID: 40769205 -
Cancer Cell
Pericancerous cross-presentation to cytotoxic T lymphocytes impairs immunotherapeutic efficacy in hepatocellular carcinoma. [Abstract]2024 Dec 9;42(12):2082-2097.e10. PMID: 39547231 -
Cell Metab
Hepatocyte-to-intestinal stem cell remote communication regulates blood glucose homeostasis. [Abstract]2026 Jul 7;38(7):1367-1384.e9. PMID: 42309059 -
Adv Mater
Calcium Phosphate Nanoparticle-Immobilized Macrophage-Derived Extracellular Vesicle Nanohybrid Facilitates Diabetic Bone Regeneration. [Abstract]2025 Oct 1:e09410. PMID: 41030201 -
Adv Mater
A T-cell Inspired Sonoporation System Enhances Low-dose X-ray-mediated Pyroptosis and Radioimmunotherapy Efficacy by Restoring Gasdermin-E Expression. [Abstract]2024 Mar 24:e2401384. PMID: 38521987 -
Nat Immunol
2019 Apr;20(4):433-446. PMID: 30804553 -
Cell Stem Cell
Glioblastoma stem cell-specific histamine secretion drives pro-angiogenic tumor microenvironment remodeling. [Abstract]2022 Nov 3;29(11):1531-1546.e7. PMID: 36265493
BAPTA-AM purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2022 Nov 3;29(11):1531-1546.e7. [Abstract]
When HBMEC cells are pre-treated with BAPTA-AM (for 5, 15, 30, 60, 90 min), histamine-induced rapid activation of NF-kB signaling is significantly inhibited.
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J Extracell Vesicles
Rapid production method with increased yield of high-purity extracellular vesicles obtained using extended mitochondrial targeting domain peptide. [Abstract]2022 Oct;11(10):e12274. PMID: 36239712 -
Bone Res
mTORC1 induces plasma membrane depolarization and promotes preosteoblast senescence by regulating the sodium channel Scn1a. [Abstract]2022 Mar 8;10(1):25. PMID: 35256591 -
Autophagy
Impaired degradation of PLCG1 by chaperone-mediated autophagy promotes cellular senescence and intervertebral disc degeneration. [Abstract]2025 Feb;21(2):352-373. PMID: 39212196 -
Autophagy
Activation of BK channels prevents diabetes-induced osteopenia by regulating mitochondrial Ca2+ and SLC25A5/ANT2-PINK1-PRKN-mediated mitophagy. [Abstract]2024 Nov;20(11):2388-2404. PMID: 38873928 -
Autophagy
2021 Apr;17(4):925-947. PMID: 32160078 -
Nat Commun
2023 Feb 6;14(1):642. PMID: 36746963 -
Nat Commun
Perfluoroalkyl substance pollutants activate the innate immune system through the AIM2 inflammasome. [Abstract]2021 May 18;12(1):2915. PMID: 34006824 -
J Adv Res
Hypericin photoactivation induces triple-negative breast cancer cells pyroptosis by targeting the ROS/CALR/Caspase-3/GSDME pathway. [Abstract]2025 Jan 25:S2090-1232(25)00059-1. PMID: 39870303 -
Redox Biol
TRPV4 drives macrophage pyroptosis via mitochondrial dysfunction and mtROS-dependent NLRP3 inflammasome activation in acute lung injury. [Abstract]2026 Jun 11:95:104255. PMID: 42314332 -
Redox Biol
Lactate-driven ATP6V1B2 lactylation triggers asthmatic inflammation by linking lysosomal dysfunction to mitochondrial ROS-dependent pyroptosis. [Abstract]2026 Mar:90:104059. PMID: 41637881 -
J Nanobiotechnology
Ion-interference amplifier nano-system enhances radiotherapy via mitochondrial dysfunction and efferocytosis Inhibition. [Abstract]2025 Nov 25;23(1):737. PMID: 41291692 -
Acta Pharm Sin B
2025 Aug;15(8):4078-4095. PMID: 40893681 -
J Exp Clin Cancer Res
CALB2 drives pancreatic cancer metastasis through inflammatory reprogramming of the tumor microenvironment. [Abstract]2024 Oct 3;43(1):277. PMID: 39358777 -
Adv Sci (Weinh)
Piezo1 Upregulation in Monocyte-Derived Macrophages Impairs Post-Myocardial Infarction Cardiac Repair via Defective Efferocytosis and Enhanced Ferroptosis. [Abstract]2025 Nov 10:e10991. PMID: 41214880 -
Adv Sci (Weinh)
2025 Aug 7:e09208. PMID: 40776529 -
Adv Sci (Weinh)
Activation of Lysosomal Retrograde Transport Triggers TPC1-IP3R1 Ca2+ Crosstalk at Lysosome-ER MCSs Leading to Lethal Depleting of ER Calcium. [Abstract]2025 Jul 25:e15313. PMID: 40709664 -
Adv Sci (Weinh)
MCU Upregulation Overactivates Mitophagy by Promoting VDAC1 Dimerization and Ubiquitination in the Hepatotoxicity of Cadmium. [Abstract]2023 Mar;10(7):e2203869. PMID: 36642847 -
Cell Death Dis
2021 Dec 14;12(12):1159. PMID: 34907173 -
Cell Death Dis
The interaction of S100A16 and GRP78 actives endoplasmic reticulum stress-mediated through the IRE1α/XBP1 pathway in renal tubulointerstitial fibrosis. [Abstract]2021 Oct 13;12(10):942. PMID: 34645789 -
Small
2025 Mar;21(12):e2408139. PMID: 40012250 -
Cancer Lett
2023 Nov 28:577:216435. PMID: 37806516 -
Cancer Lett
NCAPD2 inhibits autophagy by regulating Ca2+/CAMKK2/AMPK/mTORC1 pathway and PARP-1/SIRT1 axis to promote colorectal cancer. [Abstract]2021 Nov 1:520:26-37. PMID: 34229059 -
Int J Biol Sci
Abnormal mechanical load aggravates subchondral bone remodeling and uneven tibial plateau settlement in knee osteoarthritis via activation of osteoblast Piezo1-Ca²⁺-JAK2/STAT3 signaling. [Abstract]2026 Jan 15;22(4):1834-1851. PMID: 41694589 -
Int J Biol Sci
Melatonin modulates the hypothalamic-pituitary neuroendocrine axis to regulate physiological color change in teleost fish. [Abstract]2023 Jun 4;19(9):2914-2933. PMID: 37324950 -
Cell Commun Signal
Inhibition of SGLT2 protects podocytes in diabetic kidney disease by rebalancing mitochondria-associated endoplasmic reticulum membranes. [Abstract]2024 Nov 7;22(1):534. PMID: 39511548 -
Phytomedicine
3,3'-Diindolylmethane ameliorates muscle atrophy by modulating mitochondrial function and calcium homeostasis. [Abstract]2026 Jun 11:159:158409. PMID: 42308990 -
Phytomedicine
β-mangostin triggers ER stress-mediated mitochondrial dysfunction to induce apoptosis in colorectal cancer. [Abstract]2026 May 26. PMID: 42275878 -
Phytomedicine
Curcuminoids WM03 inhibits ovarian cancer cisplatin-resistant cells proliferation and reverses cisplatin resistance by targeting DYRK2. [Abstract]2025 Jul:142:156632. PMID: 40315643 -
Mater Today Bio
Cerium-based nanoplatform for severe acute pancreatitis: Achieving enhanced anti-inflammatory effects through calcium homeostasis restoration and oxidative stress mitigation. [Abstract]2025 Jan 13:31:101489. PMID: 39906206 -
Part Fibre Toxicol
Neuroinflammation is induced by tongue-instilled ZnO nanoparticles via the Ca2+-dependent NF-κB and MAPK pathways. [Abstract]2018 Oct 19;15(1):39. PMID: 30340606
BAPTA-AM purchased from MedChemExpress. Usage Cited in: Part Fibre Toxicol. 2018 Oct 19;15(1):39. [Abstract]
Total proteins of BV2 cells are extracted, and the levels of P2X7, NF-κB, ERK and p38 signaling pathway molecules are analyzed via Western Blot.
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eGastroenterology
2026 Mar 31;4(1):e100348. PMID: 41948149 -
Cell Death Discov
2025 Jun 21;11(1):284. PMID: 40544161 -
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Proc Natl Acad Sci U S A
2025 Sep 2;122(35):e2513155122. PMID: 40880539 -
Food Res Int
Disruption of the gut microbiota induces the LCA/TRPV6 signalling axis, impairing desmosome junctions in vitamin A-deficient rats. [Abstract]2025 Dec;222(Pt 1):117656. PMID: 41267264 -
Small Methods
An Integrated Microcurrent Delivery System Facilitates Human Parathyroid Hormone Delivery for Enhancing Osteoanabolic Effect. [Abstract]2025 Mar;9(3):e2401144. PMID: 39420694 -
Int J Biol Macromol
Duck hepatitis A virus 1-encoded 2B protein disturbs ion and organelle homeostasis to promote NF-κB/NLRP3-mediated inflammatory response. [Abstract]2024 Sep 23;280(Pt 3):135876. PMID: 39322136 -
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Free Radic Biol Med
Redox switch C674 in SERCA2 triggers Ca2+-calcineurin-MCU-Drp1 cascade and pulmonary vascular remodeling. [Abstract]2025 Dec 16:244:380-394. PMID: 41412527 -
Free Radic Biol Med
V8, a lysosomotropic wogonin derivative, alleviates hepatic steatosis by modulating GDF15-dependent mitochondrial homeostasis. [Abstract]2025 Oct 23:S0891-5849(25)01299-7. PMID: 41138960 -
EMBO Mol Med
5-HT regulates resistance to aumolertinib by attenuating ferroptosis in lung adenocarcinoma. [Abstract]2025 Oct;17(10):2586-2611. PMID: 40897859 -
EMBO Mol Med
PGF2α facilitates pathological retinal angiogenesis by modulating endothelial FOS-driven ELR+ CXC chemokine expression. [Abstract]2023 Jan 11;15(1):e16373. PMID: 36511116 -
Clin Transl Med
Low-glucose-sensitive TRPC6 dysfunction drives hypoglycemia-induced cognitive impairment in diabetes. [Abstract]2020 Oct;10(6):e205. PMID: 33135341 -
Cell Rep
Macrophage glycine transporter supports IL-1β production by modulating the AKT1/mTOR/NLRP3 pathway. [Abstract]2026 Jan 3;45(1):116683. PMID: 41485223 -
Cell Rep
circATF6 triggers calcium overload to synergize with EGFR-TKI in non-small cell lung cancer via modulation of proteostasis. [Abstract]2025 Dec 5;44(12):116659. PMID: 41351835 -
Aging Cell
Piezo1 exacerbates inflammation-induced cartilaginous endplate degeneration by activating mitochondrial fission via the Ca2+/CaMKII/Drp1 axis. [Abstract]2025 Apr;24(4):e14440. PMID: 39610146 -
Cell Rep
The N-degron pathway mediates the autophagic degradation of cytosolic mitochondrial DNA during sterile innate immune responses. [Abstract]2024 Dec 21;44(1):115094. PMID: 39709605 -
Chin Med
Shexiang Tongxin Dropping Pills targeting Piezo1/Ca2+/NLRP3 axis attenuates vascular endothelial inflammation. [Abstract]2026 Apr 29;21(1):123. PMID: 42057150 -
Virulence
Integrated transcriptomic and proteomic analysis reveals inflammatory activation and blood-brain barrier disruption during meningitis-associated extraintestinal pathogenic Escherichia coli infection. [Abstract]2026 Dec;17(1):2670939. PMID: 42115138 -
Virulence
2025 Dec;16(1):2490208. PMID: 40202859 -
Virulence
The T-type voltage-gated Ca2+ channel CaV3.1 involves in the disruption of respiratory epithelial barrier induced by Pasteurella multocida toxin. [Abstract]2025 Dec;16(1):2466482. PMID: 39950866 -
Virulence
Newcastle disease virus acquires phosphatidylserine through the budding process to enhance infectivity. [Abstract]2025 Oct 28:2580150. PMID: 41147668 -
NanoImpact
CdTe quantum dots induce apoptosis in RSC96 cells by disrupting calcium homeostasis and triggering subcellular structural dysfunction. [Abstract]2025 Jul:39:100572. PMID: 40527434 -
J Agric Food Chem
Apigenin-Mediated ESCRT-III Activation and Mitophagy Alleviate LPS-Induced Necroptosis in Renal Cells. [Abstract]2025 Apr 10. PMID: 40211127 -
CNS Neurosci Ther
High Shear Stress-Induced Endothelial Piezo1 Downregulation Promotes Intracranial Aneurysm Formation via the PDGF-BB/PDGFRβ Paracrine Signaling Pathway. [Abstract]2025 Dec;31(12):e70715. PMID: 41457305 -
Ecotoxicol Environ Saf
MicroRNA-129-1-3p protects chicken granulosa cells from cadmium-induced apoptosis by down-regulating the MCU-mediated Ca2+ signaling pathway. [Abstract]2024 Jan 1:269:115906. PMID: 38176135 -
Ecotoxicol Environ Saf
The rare-earth yttrium induces cell apoptosis and autophagy in the male reproductive system through ROS-Ca2+-CamkII/Ampk axis. [Abstract]2023 Sep 15:263:115262. PMID: 37480693 -
Ecotoxicol Environ Saf
Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium-mediated RhoA/ROCK signaling and myosin light chain kinase. [Abstract]2023 Jun 1:257:114940. PMID: 37099960 -
Biochem Pharmacol
Targeting neuropilin-1 attenuates 4-hydroxytamoxifen-induced stemness and sensitizes ERα-re-expressing TNBC to tamoxifen. [Abstract]2026 Jun 12;251(Pt 2):118163. PMID: 42285368 -
Biochem Pharmacol
Effects of G protein-coupled estrogen receptor on hydrogen sulfide production and cystathionine γ-lyase expression in human endothelial cells. [Abstract]2025 Sep 19;242(Pt 3):117354. PMID: 40976431 -
Biochem Pharmacol
3,3'-Diindolylmethane promotes bone formation - A assessment in MC3T3-E1 cells and zebrafish. [Abstract]2024 Nov 10;230(Pt 3):116618. PMID: 39528071 -
Cell Mol Life Sci
Selenium-SelK-GPX4 axis protects nucleus pulposus cells against mechanical overloading-induced ferroptosis and attenuates senescence of intervertebral disc. [Abstract]2024 Jan 22;81(1):49. PMID: 38252317 -
Life Sci
Berberine ameliorates oxidative stress-induced apoptosis by modulating ER stress and autophagy in human nucleus pulposus cells. [Abstract]2019 Jul 1:228:85-97. PMID: 31047897 -
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RSC Adv
Ginsenoside Rk1 inhibits cell proliferation and promotes apoptosis in lung squamous cell carcinoma by calcium signaling pathway. [Abstract]2019 Aug 13;9(43):25107-25118. PMID: 35528653 -
Cells
Activin A, a Novel Chemokine, Induces Mouse NK Cell Migration via AKT and Calcium Signaling. [Abstract]2024 Apr 23;13(9):728. PMID: 38727264 -
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Novel recombinant protein PK5-Gal-3C enhances the anti-tumor activity of T cells via binding with TRPV2. [Abstract]2026 May 8;75(7):175. PMID: 42101653 -
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Oncotarget
HBx regulates fatty acid oxidation to promote hepatocellular carcinoma survival during metabolic stress. [Abstract]2016 Feb 9;7(6):6711-26. PMID: 26744319
BAPTA-AM purchased from MedChemExpress. Usage Cited in: Oncotarget. 2016 Feb 9;7(6):6711-26. [Abstract]
Under conditions of glucose limitation, BAPTA-AM treatment prevents HBx-induced phosphorylation of AMPK and ACC (A). This inhibitory effect is also observed in HBV-infected HepG2.2.15 cells in a concentration-dependent manner (B), implying that HBx-mediated mitochondrial calcium channels are required for activation of the AMPK pathway under such stressful conditions.
Solvent & Solubility
DMSO : 100 mg/mL (130.77 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.27 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: 2.5 mg/mL (3.27 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
Neuronal injury is quantitatively estimated by measuring lactate dehydrogenase (LDH) released from damaged cells into the bathing medium 24- or 48-hr after the 10 μM BAPTA/AM treatment. The morphological findings are confirmed by staining with neuron-specific enolase (NSE) antibody and tryphan blue[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
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Data Sheet (282 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Tang Q, et al. The membrane permeable calcium chelator BAPTA-AM directly blocks human ether a-go-go-related gene potassium channels stably expressed in HEK 293 cells. Biochem Pharmacol. 2007 Dec 3;74(11):1596-607. [Content Brief]
[2]. Wie MB, et al. BAPTA/AM, an intracellular calcium chelator, induces delayed necrosis by lipoxygenase-mediated free radicals in mouse cortical cultures. Prog Neuropsychopharmacol Biol Psychiatry. 2001 Nov;25(8):1641-59. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3077 mL | 6.5387 mL | 13.0774 mL | 32.6934 mL |
| 5 mM | 0.2615 mL | 1.3077 mL | 2.6155 mL | 6.5387 mL | |
| 10 mM | 0.1308 mL | 0.6539 mL | 1.3077 mL | 3.2693 mL | |
| 15 mM | 0.0872 mL | 0.4359 mL | 0.8718 mL | 2.1796 mL | |
| 20 mM | 0.0654 mL | 0.3269 mL | 0.6539 mL | 1.6347 mL | |
| 25 mM | 0.0523 mL | 0.2615 mL | 0.5231 mL | 1.3077 mL | |
| 30 mM | 0.0436 mL | 0.2180 mL | 0.4359 mL | 1.0898 mL | |
| 40 mM | 0.0327 mL | 0.1635 mL | 0.3269 mL | 0.8173 mL | |
| 50 mM | 0.0262 mL | 0.1308 mL | 0.2615 mL | 0.6539 mL | |
| 60 mM | 0.0218 mL | 0.1090 mL | 0.2180 mL | 0.5449 mL | |
| 80 mM | 0.0163 mL | 0.0817 mL | 0.1635 mL | 0.4087 mL | |
| 100 mM | 0.0131 mL | 0.0654 mL | 0.1308 mL | 0.3269 mL |
BAPTA-AM Related Classifications
HY-100545 Related Classifications
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