1. Biochemical Assay Reagents


Cat. No.: HY-100545 Purity: 99.60%
Handling Instructions

BAPTA-AM is an intracellular calcium chelator.

For research use only. We do not sell to patients.

BAPTA-AM Chemical Structure

BAPTA-AM Chemical Structure

CAS No. : 126150-97-8

Size Price Stock Quantity
10 mM * 1 mL in DMSO USD 71 In-stock
Estimated Time of Arrival: December 31
5 mg USD 60 In-stock
Estimated Time of Arrival: December 31
10 mg USD 84 In-stock
Estimated Time of Arrival: December 31
50 mg USD 336 In-stock
Estimated Time of Arrival: December 31
100 mg USD 576 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

    BAPTA-AM purchased from MCE. Usage Cited in: Oncotarget. 2016 Feb 9;7(6):6711-26.

    Under conditions of glucose limitation, BAPTA-AM treatment prevents HBx-induced phosphorylation of AMPK and ACC (A). This inhibitory effect is also observed in HBV-infected HepG2.2.15 cells in a concentration-dependent manner (B), implying that HBx-mediated mitochondrial calcium channels are required for activation of the AMPK pathway under such stressful conditions.

    BAPTA-AM purchased from MCE. Usage Cited in: Part Fibre Toxicol. 2018 Oct 19;15(1):39.

    Total proteins of BV2 cells are extracted, and the levels of P2X7, NF-κB, ERK and p38 signaling pathway molecules are analyzed via Western Blot.
    • Biological Activity

    • Protocol

    • Technical Information

    • Purity & Documentation

    • References


    BAPTA-AM is an intracellular calcium chelator.

    IC50 & Target

    calcium chelator[1]

    In Vitro

    The permeable calcium chelator BAPTA/AM is known to prevent free radical-mediated toxicity promote apoptosis in non-neuronal cells and produce a beneficial effect in neuronal cells by protecting neurons from ischemic damage. In addition, it has been suggested that BAPTA/AM induces a late, but not early, increase of intracellular calcium in I-IL-60 neoplastic cells. Mixed cortical cell cultures (DIV 13-16) exposed to 10μM BAPTA/AM for 24- or 48-hr show moderate (45-70%) neuronal injury as evaluated by increased LDH release into the bathing medium after 24-48-hr. Exposure of cortical cultures to 3-10 μM BAPTA/AM for 48-hr evoke dose-dependent neuronal damage[1].

    Solvent & Solubility
    In Vitro: 

    10 mM in DMSO

    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.3077 mL 6.5387 mL 13.0774 mL
    5 mM 0.2615 mL 1.3077 mL 2.6155 mL
    10 mM 0.1308 mL 0.6539 mL 1.3077 mL
    *Please refer to the solubility information to select the appropriate solvent.
    Cell Assay

    Neuronal injury is quantitatively estimated by measuring lactate dehydrogenase (LDH) released from damaged cells into the bathing medium 24- or 48-hr after the 10 μM BAPTA/AM treatment. The morphological findings are confirmed by staining with neuron-specific enolase (NSE) antibody and tryphan blue[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight




    CAS No.




    Powder -20°C 3 years
      4°C 2 years
    In solvent -80°C 6 months
      -20°C 1 month

    Room temperature in continental US; may vary elsewhere

    Purity: 99.60%

    • Molarity Calculator

    • Dilution Calculator

    The molarity calculator equation

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass   Concentration   Volume   Molecular Weight *
    = × ×

    The dilution calculator equation

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
    × = ×
    C1   V1   C2   V2

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product name



    Applicant name *


    Email address *

    Phone number *


    Organization name *

    Country or Region *


    Requested quantity *


    Bulk Inquiry

    Inquiry Information

    Product Name:
    Cat. No.:


    Cat. No.: HY-100545