2. Academic Validation
  3. Porcine deltacoronavirus (PDCoV) modulates calcium influx to favor viral replication

Porcine deltacoronavirus (PDCoV) modulates calcium influx to favor viral replication

  • Virology. 2020 Jan 2;539:38-48. doi: 10.1016/j.virol.2019.10.011.
Dongcheng Bai 1,  Liurong Fang 2,  Sijin Xia 1,  Wenting Ke 1,  Jing Wang 1,  Xiaoli Wu 1,  Puxian Fang 1,  Shaobo Xiao 1
Affiliations

Affiliations

  • 1 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China.
  • 2 State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, China; Key Laboratory of Preventive Veterinary Medicine in Hubei Province, The Cooperative Innovation Center for Sustainable Pig Production, Wuhan, 430070, China. Electronic address: [email protected].
PMID: 31670218 DOI: 10.1016/j.virol.2019.10.011
Abstract

Ionic calcium (Ca2+) is a versatile intracellular second messenger that plays important roles in cellular physiological and pathological processes. Porcine deltacoronavirus (PDCoV) is an emerging enteropathogenic coronavirus that causes serious vomiting and diarrhea in suckling piglets. In this study, the role of Ca2+ to PDCoV Infection was investigated. PDCoV Infection was found to upregulate intracellular Ca2+ concentrations of IPI-2I cells. Chelating extracellular Ca2+ by EGTA inhibited PDCoV replication, and this inhibitory effect was overcome by replenishment with CaCl2. Treatment with Ca2+ channel blockers, particularly the L-type Ca2+ channel blocker diltiazem hydrochloride, inhibited PDCoV Infection significantly. Mechanistically, diltiazem hydrochloride reduces PDCoV Infection by inhibiting the replication step of the viral replication cycle. Additionally, knockdown of CACNA1S, the L-type Ca2+ voltage-gated channel subunit, inhibited PDCoV replication. The combined results demonstrate that PDCoV modulates calcium influx to favor its replication.

Keywords

Ca(2+) channel blocker; Ca(2+) influx; Porcine deltacoronavirus; Viral replication.

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