Diltiazem hydrochloride
Based on 11 publication(s) in Google Scholar
Diltiazem hydrochloride (CRD-401) is a calcium antagonist and an L-type calcium channel inhibitor. Diltiazem hydrochloride inhibits Ca2+ mobilization/influx, norepinephrine-induced contraction, and depolarization. Diltiazem hydrochloride produces coronary vasodilation and renal vasodilation; it inhibits sodium reabsorption in the distal nephron and increases urine flow, sodium and potassium excretion, Inulin (HY-N7075) and PAH clearance, and free water clearance. Diltiazem hydrochloride is used in research on cardiovascular diseases, kidney diseases, and inflammation.
For research use only. We do not sell to patients.
- Purity : 99.47%
- CAS No.: 33286-22-5
- Formula: C22H27ClN2O4S
- Molecular Weight:450.98
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
-
HY-B0284
NifedipineCalcium Channel Antagonist -
HY-A0064
Verapamil hydrochlorideCalcium Channel Blocker -
HY-B0309
FelodipineCalcium Channel Antagonist -
HY-100831
YM-58483CRAC Channel/SOCE Blocker -
HY-B0317B
Amlodipine besylateCalcium Channel Blocker -
HY-16569
ColchicineTubulin Inhibitor -
HY-15772
OsimertinibMutant-Selective EGFR Inhibitor -
HY-17402
NisoldipineCalcium Channel Blocker -
HY-18739
Phorbol 12-myristate 13-acetatePKC Activator -
HY-100579
Ferrostatin-1Ferroptosis Inhibitor -
HY-W009724
2-Aminoethyl diphenylborinateIP3R Inhibitor -
HY-12507
GSK-7975ACRAC Channel Inhibitor
Publications Citing Use of MedChemExpress (MCE) Diltiazem hydrochloride
More- Nat Commun. 2024 Jun 19;15(1):5230. [Abstract]
- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- J Gen Physiol. 2026 May 4;158(3):e202513819. [Abstract]
- Immunobiology. 2026 Sep;231(5):153237.
- Vet Sci. 2025 Sep 5;12(9):864. [Abstract]
- J Cardiovasc Transl Res. 2023 Aug;16(4):758-767. [Abstract]
- Pharmacol Res Perspect. 2021 Oct;9(5):e00879. [Abstract]
- Pharmacol Res Perspect. 2020 Apr;8(2):e00575. [Abstract]
- Virology. 2020 Jan 2:539:38-48. [Abstract]
- medRxiv. 2025 May 21.
Customer Validation & Images
-
Bio/Physico-chemical Assay
All Calcium Channel Isoforms
More
Biological Activity
Description
IC50 & Target
[4]|
L-type calcium channel |
In Vitro
Diltiazem hydrochloride reduces depolarization-induced contraction in voltage-clamped rabbit main pulmonary artery[3].
Diltiazem hydrochloride reduces norepinephrine-induced contraction of rabbit main pulmonary artery and inhibits norepinephrine-induced depolarization[3].
Diltiazem hydrochloride increases the K+-induced mechanical threshold in rabbit main pulmonary arteries and reduces K+-induced contraction[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Diltiazem hydrochloride (10 μg/kg/min; continuous intrarenal arterial infusion) increases sodium excretion even when renal blood flow is maintained constant by aortic clamping; and it reduces sodium reabsorption in the distal nephron[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 33286-22-5
-
Appearance Solid
-
Molecular Weight 450.98
-
Formula C22H27ClN2O4S
-
Color White to off-white
-
SMILES
CN(C)CCN1C2=CC=CC=C2S[C@@H](C3=CC=C(OC)C=C3)[C@@H](OC(C)=O)C1=O.Cl
-
Synonyms
CRD-401
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (11)
-
Journal Impact Factor
-
Most Recent
-
Nat Commun
Small molecule in situ resin capture provides a compound first approach to natural product discovery. [Abstract]2024 Jun 19;15(1):5230. PMID: 38898025 -
ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
J Gen Physiol
2026 May 4;158(3):e202513819. PMID: 41784565 -
-
Vet Sci
2025 Sep 5;12(9):864. PMID: 41012789 -
J Cardiovasc Transl Res
2023 Aug;16(4):758-767. PMID: 36715820
Diltiazem hydrochloride purchased from MedChemExpress. Usage Cited in: J Cardiovasc Transl Res. 2023 Aug;16(4):758-767. [Abstract]
Representative beating curves of NRVC exposed to Diltiazem (100 nM) or PBS for 24 h (n = 3) and quantitative analysis of the relative beating rate and intensity of NRVC exposed to Diltiazem (100 nM) or PBS for 0 h and 24 h (n = 3).
-
Pharmacol Res Perspect
Effects of membrane transport activity and cell metabolism on the unbound drug concentrations in the skeletal muscle and liver of drugs: A microdialysis study in rats. [Abstract]2021 Oct;9(5):e00879. PMID: 34628723 -
Pharmacol Res Perspect
Applicability of free drug hypothesis to drugs with good membrane permeability that are not efflux transporter substrates: A microdialysis study in rats. [Abstract]2020 Apr;8(2):e00575. PMID: 32266794 -
Virology
2020 Jan 2:539:38-48. PMID: 31670218 -
Solvent & Solubility
In Vitro:
H2O : 33.33 mg/mL (73.91 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 100 mg/mL (221.74 mM); Clear solution; Need ultrasonic
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
-
Data Sheet (297 KB)
-
SDS (419 KB)
- English - EN (419 KB)
- Français - FR (419 KB)
- Deutsch - DE (419 KB)
- Norwegian - NO (419 KB)
- Español - ES (419 KB)
- Swedish - SV (419 KB)
- Italian - IT (419 KB)
- Korean - KR (419 KB)
- Portuguese - PT (419 KB)
-
Handling Instructions (2659 KB)
References
[3]. Ito Y, et al. The effects of diltiazem (CRD-401) on the membrane and mechanical properties of vascular smooth muscles of the rabbit. British journal of pharmacology. 1978 Dec;64(4):503-10. [Content Brief]
[4]. Mieth A, et al. L-type calcium channel inhibitor diltiazem prevents aneurysm formation by blood pressure-independent anti-inflammatory effects. Hypertension. 2013 Dec;62(6):1098-104. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 2.2174 mL | 11.0870 mL | 22.1739 mL | 55.4348 mL |
| 5 mM | 0.4435 mL | 2.2174 mL | 4.4348 mL | 11.0870 mL | |
| 10 mM | 0.2217 mL | 1.1087 mL | 2.2174 mL | 5.5435 mL | |
| 15 mM | 0.1478 mL | 0.7391 mL | 1.4783 mL | 3.6957 mL | |
| 20 mM | 0.1109 mL | 0.5543 mL | 1.1087 mL | 2.7717 mL | |
| 25 mM | 0.0887 mL | 0.4435 mL | 0.8870 mL | 2.2174 mL | |
| 30 mM | 0.0739 mL | 0.3696 mL | 0.7391 mL | 1.8478 mL | |
| 40 mM | 0.0554 mL | 0.2772 mL | 0.5543 mL | 1.3859 mL | |
| 50 mM | 0.0443 mL | 0.2217 mL | 0.4435 mL | 1.1087 mL | |
| 60 mM | 0.0370 mL | 0.1848 mL | 0.3696 mL | 0.9239 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Diltiazem hydrochloride Related Classifications
HY-14656 Related Classifications
Powered by Bioz