2. Academic Validation
  3. Developmental toxicity and transcriptome analysis of 4-epianhydrotetracycline to zebrafish (Danio rerio) embryos

Developmental toxicity and transcriptome analysis of 4-epianhydrotetracycline to zebrafish (Danio rerio) embryos

  • Sci Total Environ. 2020 Sep 10;734:139227. doi: 10.1016/j.scitotenv.2020.139227.
Meng Wang 1,  Xiaoping Chen 1,  Run Zhang 1,  Jianfu Zhao 1,  Changming Yang 1,  Lingling Wu 2
Affiliations

Affiliations

  • 1 Key Laboratory of Yangtze River Water Environment of the Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China.
  • 2 Key Laboratory of Yangtze River Water Environment of the Ministry of Education, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, PR China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, PR China. Electronic address: [email protected].
PMID: 32450397 DOI: 10.1016/j.scitotenv.2020.139227
Abstract

As a primary degradation by-product of Tetracycline (TC), 4-Epianhydrotetracycline (4-EATC) has been detected frequently in the aquatic environment, which may pose a potential environmental risk to aquatic organisms. Up to now, however, the toxicology study on 4-EATC to aquatic organisms is limited. In the present study, in order to better understand the toxic mechanism of 4-EATC, developmental toxicity including lethal and sublethal effects of 4-EATC and TC were investigated. The results showed that the developmental toxicity of 4-EATC to zebrafish embryos was stronger than that of TC. The 96 h LC50 value of 4-EATC to zebrafish embryos was 29.13 mg/L. Malformations seemed to be the most sensitive sublethal endpoint of 4-EATC exposure, and the 96 h EC50 value was 8.57 mg/L. Transcriptome response of 4-EATC to zebrafish embryos was determined. The results showed that 430 different expression genes (DEGs) caused by 4-EATC, and most enriched in tryptophan (TRP) metabolism pathway. Annotation of DEGs in the TRP metabolism demonstrated that expression of 4 gene products in tryptophan metabolized along the kynurenine (KYN) pathway were changed. Disorder of TRP catabolism in KYN pathway was a potential mechanism of 4-EATC toxicity to zebrafish embryos.

Keywords

4-Epianhydrotetracycline; Degradation by-product; Developmental toxicity; Toxic mechanism; Transcriptomics; Zebrafish embryos.

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