4-Epianhydrotetracycline hydrochloride
Based on 1 Customer Validation
4-Epianhydrotetracycline hydrochloride is a major intermediate product of Tetracycline (HY-A0107). 4-Epianhydrotetracycline hydrochloride shows lethal effects and induces cell apoptosis of zebrafish embryos. 4-Epianhydrotetracycline hydrochloride inhibits Shewanella, E. coli and P. aeruginosa with MIC values of 2, 1 and 64 mg/L, respectively.
For research use only. We do not sell to patients.
- Purity: 90.21%
- CAS No.: 4465-65-0
- Formula: C22H23ClN2O7
- Molecular Weight:462.88
-
Storage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
All Antibiotic Isoforms
More
Biological Activity
|
Tetracycline |
4-Epianhydrotetracycline hydrochloride (0-20 mg/L; 96 h) significantly enhances ROS levels, and malondialdehyde (MDA) concentration and protein carbonyl (PC) content with increasing concentration of 5-20.0 mg/L[1]. 4-Epianhydrotetracycline hydrochloride (0-20 mg/L; 96 h) dose-dependently affects SOD activities with increasing first and then decreasing, and at a dose of 2.50 mg/L SOD activity reached the highest value[1]. 4-Epianhydrotetracycline hydrochloride (0-20 mg/L; 96 h) induces cell apoptosis[1]. 4-Epianhydrotetracycline hydrochloride (0-100 mg/L) shows lethal effects of zebrafish embryos with an LC50 value of 29.13 mg/L at 96 h[2]. 4-Epianhydrotetracycline hydrochloride increases zebrafish embryos malformation with an EC50 value of 8.57 mg/L at 96 h[2]. 4-Epianhydrotetracycline hydrochloride inhibits the heart rate (10-20 mg/L) and shows delayed hatching effects (5-20.0 mg/L) [2]. 4-Epianhydrotetracycline hydrochloride (0-20 mg/L) shows inhibitory effects to Shewanella, E. coli and P. aeruginosa with MIC values of 2, 1 and 64 mg/L, respectively[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:Zebrafish embryos
-
Concentration:0, 1.25, 2.5, 5, 10 and 20 mg/L
-
Incubation Time:96 hours
-
Result:Dose-dependently induced cell apoptosis with 66.67% zebrafish embryos showed apoptosis phenomenon at a dose of 20.0 mg/L.
Chemical Information
-
CAS No. 4465-65-0
-
Appearance Solid
-
Molecular Weight 462.88
-
Formula C22H23ClN2O7
-
Color Orange to red
-
SMILES
O[C@@](C(C1=C(C2=C3O)O)=O)(C(C(C(N)=O)=C4O)=O)[C@](CC1=C(C2=CC=C3)C)([H])[C@H]4N(C)C.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
DMSO : 50 mg/mL (108.02 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 1.25 mg/mL (2.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1.25 mg/mL (2.70 mM); Clear solution
This protocol yields a clear solution of ≥ 1.25 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (12.5 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
-
Data Sheet (278 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Wang M, et al. Effects of 4-epianhydrotetracycline on oxidative stress in zebrafish (Danio rerio) embryos. Sci Total Environ. 2021 Nov 20;796:149047. [Content Brief]
[2]. Wang M, et al. Developmental toxicity and transcriptome analysis of 4-epianhydrotetracycline to zebrafish (Danio rerio) embryos. Sci Total Environ. 2020 Sep 10;734:139227. [Content Brief]
[3]. Long S, et al. Toxicity of tetracycline and its transformation products to a phosphorus removing Shewanella strain. Chemosphere. 2020 May;246:125681. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.1604 mL | 10.8019 mL | 21.6039 mL | 54.0097 mL |
| 5 mM | 0.4321 mL | 2.1604 mL | 4.3208 mL | 10.8019 mL | |
| 10 mM | 0.2160 mL | 1.0802 mL | 2.1604 mL | 5.4010 mL | |
| 15 mM | 0.1440 mL | 0.7201 mL | 1.4403 mL | 3.6006 mL | |
| 20 mM | 0.1080 mL | 0.5401 mL | 1.0802 mL | 2.7005 mL | |
| 25 mM | 0.0864 mL | 0.4321 mL | 0.8642 mL | 2.1604 mL | |
| 30 mM | 0.0720 mL | 0.3601 mL | 0.7201 mL | 1.8003 mL | |
| 40 mM | 0.0540 mL | 0.2700 mL | 0.5401 mL | 1.3502 mL | |
| 50 mM | 0.0432 mL | 0.2160 mL | 0.4321 mL | 1.0802 mL | |
| 60 mM | 0.0360 mL | 0.1800 mL | 0.3601 mL | 0.9002 mL | |
| 80 mM | 0.0270 mL | 0.1350 mL | 0.2700 mL | 0.6751 mL | |
| 100 mM | 0.0216 mL | 0.1080 mL | 0.2160 mL | 0.5401 mL |
4-Epianhydrotetracycline hydrochloride Related Classifications
HY-136439 Related Classifications
Powered by Bioz