2. Academic Validation
  3. Extracts and Steroids from the Edible Mushroom Hypholoma lateritium Exhibit Anti-Inflammatory Properties by Inhibition of COX-2 and Activation of Nrf2

Extracts and Steroids from the Edible Mushroom Hypholoma lateritium Exhibit Anti-Inflammatory Properties by Inhibition of COX-2 and Activation of Nrf2

  • Chem Biodivers. 2020 Sep;17(9):e2000391. doi: 10.1002/cbdv.202000391.
Attila Ványolós 1 2,  Bożena Muszyńska 3,  Bayar Chuluunbaatar 1,  Joanna Gdula-Argasińska 4,  Katarzyna Kała 3,  Judit Hohmann 1 5
Affiliations

Affiliations

  • 1 Department of Pharmacognosy, Interdisciplinary Excellence Center, University of Szeged, Eötvös u. 6., H-6720 Szeged, Hungary.
  • 2 Department of Pharmacognosy, Semmelweis University, Üllői u. 26, H-1085, Budapest, Hungary.
  • 3 Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian, University Medical College, 9 Medyczna Street, 30-688, Kraków, Poland.
  • 4 Department of Radioligands, Faculty of Pharmacy, Jagiellonian University Medical College, 9 Medyczna Street, 30-688, Kraków, Poland.
  • 5 Interdisciplinary Center of Natural Products, University of Szeged, Eötvös u. 6., H-6720, Szeged, Hungary.
PMID: 32558141 DOI: 10.1002/cbdv.202000391
Abstract

Hypholoma lateritium is an edible macrofungus with a common distribution in Europe, North America, and the Far East. The aim of this study was to investigate the potential anti-inflammatory effects of H. lateritium extracts and its isolated steroids: fasciculic acid B, fasciculol E, fasciculol C, lanosta-7,9(11)-diene-12β,21α-epoxy-2α,3β,24β,25-tetraol, fasciculol F, and demethylincisterol A2. Organic (hexane, chloroform and 50 % methanol) and water extracts of H. lateritium were subjected to in vitro assays to determine pro-inflammatory protein levels, such as cyclooxygenase-2 (COX-2), cytosolic prostaglandin E2 synthase (cPGES), and antioxidant nuclear factor (erythroid-derived 2)-like 2 (Nrf2). Fungal extracts demonstrated significant activities on pro-inflammatory protein levels with minor differences among the activities of the fractions of different polarities. All the compounds proved to exert notable inhibitory properties on COX-2 and were capable to stimulate the Nrf2 pathway. Fungal extracts and the compounds exerted no cytotoxic activities on RAW 264.7 cells.

Keywords

COX-2; Hypholoma lateritium; Nrf2; anti-inflammatory activity; steroids.

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