1. Academic Validation
  2. Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats

Ketogenic diet aggravates cardiac remodeling in adult spontaneously hypertensive rats

  • Nutr Metab (Lond). 2020 Oct 26;17:91. doi: 10.1186/s12986-020-00510-7.
Yuehua You  # 1 Yongzheng Guo  # 1 Ping Jia 1 Biaobiao Zhuang 1 Yu Cheng 2 Hongpei Deng 2 Xiaowen Wang 3 Cheng Zhang 3 Suxin Luo 1 Bi Huang 1
Affiliations

Affiliations

  • 1 Division of Cardiology, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 China.
  • 2 Institute of Life Science, Chongqing Medical University, Chongqing, 400016 China.
  • 3 Department of Cardiothoracic Surgery, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016 China.
  • # Contributed equally.
Abstract

Background: Ketogenic diet (KD) has been proposed to be an effective lifestyle intervention in metabolic syndrome. However, the effects of KD on cardiac remodeling have not been investigated. Our aim was to investigate the effects and the underling mechanisms of KD on cardiac remodeling in spontaneously hypertensive rats (SHRs).

Methods: 10-week-old spontaneously hypertensive rats were subjected to normal diet or ketogenic diet for 4 weeks. Then, their blood pressure and cardiac remodeling were assessed. Cardiac fibroblasts were isolated from 1- to 3-day-old neonatal pups. The cells were then cultured with ketone body with or without TGF-β to investigate the mechanism in vitro.

Results: 4 weeks of KD feeding aggravated interstitial fibrosis and cardiac remodeling in SHRs. More interestingly, ketogenic diet feeding increased the activity of mammalian target of rapamyoin (mTOR) complex 2 pathway in the heart of SHRs. In addition, β-hydroxybutyrate strengthened the progression of TGF-β-induced fibrosis in isolated cardiac fibroblasts. mTOR inhibition reversed this effect, indicating that ketone body contributes to cardiac fibroblasts via mTOR pathway.

Conclusions: These data suggest that ketogenic diet may lead to adverse effects on the remodeling in the hypertensive heart, and they underscore the necessity to fully evaluate its reliability before clinical use.

Keywords

Cardiac fibrosis; Hypertension; Ketogenic diet; mTOR pathway; mTORC2.

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