1. Academic Validation
  2. Loading of doxorubicin on poly(methyl methacrylate-co-methacrylic acid) nanoparticles and release study

Loading of doxorubicin on poly(methyl methacrylate-co-methacrylic acid) nanoparticles and release study

  • J Biomater Sci Polym Ed. 2021 Jun;32(9):1107-1124. doi: 10.1080/09205063.2021.1900652.
Roberto López-Muñoz 1 María Esther Treviño 1 Fabiola Castellanos 1 Graciela Morales 1 Oliverio Rodríguez-Fernández 1 Santiago Saavedra 2 Angel Licea-Claverie 3 Hened Saade 1 Francisco Javier Enríquez-Medrano 1 Raúl Guillermo López 1
Affiliations

Affiliations

  • 1 Centro de Investigación en Química Aplicada, Saltillo, CH, México.
  • 2 Facultad de Ciencias Biológicas, Laboratorio de Inmunología y Virología, Universidad Autónoma de Nuevo León, Nuevo León, México.
  • 3 Centro de Graduados e Investigación en Química, Tecnológico Nacional de México/Instituto Tecnológico de Tijuana, Tijuana, BC, Mexico.
Abstract

Nanoparticles (NP) of 12.7 nm in diameter of the poly(methyl methacrylate (MMA)-co-methacrylic acid (MAA)) copolymer were prepared. 13C-NMR results showed a MMA:MAA molar ratio of 0.64:0.36 in the copolymer, which is similar to the poly(MMA-co-MAA) commercially known as the FDA approved Eudragit S100 (0.67:0.33). The NP prepared in this study were loaded at pH 5 with varying amounts (from 0.54 to 6.91%) of doxorubicin (DOX), an antineoplastic drug. 1H-NMR results indicated the electrostatic interactions between the ionized carboxylic groups of the MAA units in the copolymer and the proton of the glycosidic amine in DOX. Measurements by QLS and TEM indicated that the loading destabilizes the NP, and that for increase stability, they aggregate in a reversible way, forming aggregates with a diameter up to 99.5 nm at a DOX load of 6.91%. The analysis of drug release data at pH 7.4 showed that loaded NP with at least 4.38% DOX release the drug very slowly and follows the Higuchi model; the former suggests that they could remain for long periods in the bloodstream to reach and destroy Cancer cells.

Keywords

Eudragit S100; Poly(methyl methacrylate-co-methacrylic acid) latexes; doxorubicin-loaded nanoparticles.

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